Since the isolation of artemisinin 32 years ago, it has been analyzed by different chromatographic techniques. This work compared the analysis of artemisinin from crude plant samples by GC with flame ionization detection (GC-FID) and HPLC with evaporative light scattering detector (HPLC-ELSD). Data is also presented indicating that GC is suitable for the quantification of two of artemisinin pre...

Abstract A new precise, selective and reliable reversed phase high performance liquid chromatographic (RP-HPLC) method has been developed validated for the determination of Methyl paraben sodium (MPS) Propyl (PPS) (preservatives) in Iron protein succinylate syrup. Optimized conditions were; Methanol: Water (65: 35) as mobile phase, UV/Vis detector at wavelength 254 nm flow rate was set 1.3 ml m...

a special type of silica-based columns has been recently introduced into the market which is called narrow-bore columns. they have lower internal volume than the standard high-performance liquid chromatography (hplc) columns and thus reduce the solvent consumption by almost 80%.    a simple, accurate and environmentally friendly reversed phase- hplc (rp-hplc method) which could be used in fast ...

A sensitive high-performance liquid chromatographic (HPLC) method is described for the determination of posaconazole in human plasma/serum. The method is based on a single dilution step by treating the sample with methanol, and followed by the direct injection of the sample into the HPLC system. Posaconazole and internal standard ketoconazole in the methanol extract are subsequently analyzed by...

The aim of this study was to compare system efficiency and analysis duration regarding the solvent consumption and system maintenance in high-pressure liquid chromatography (HPLC) and ultra high-pressure liquid chromatography (UHPLC). In a case-control study, standard solutions of 7 benzodiazepines (BZs) and 73 biological samples such as urine, tissue, stomach content, and bile that screened po...

Background and objectives: The importance of Cassia angustifulia is due to its laxative properties. A large number of sennosides have been reported in senna but sennoside A and B have been reported as the main causes of its properties. The present study includes the analysis of the effective ingredients of sennosides containing senna products in Iranian pharmaceutical ...

We developed an ultrasensitive and highly selective method to quantify iodate (IO3–) in iodized salt biological samples. In the high-performance liquid chromatography (HPLC) system, IO3– was injected with sampler reduced iodide (I–) online using a Coulomb detector (CD) as pre-column reactor, retained by C18 column help of ion pairs, then determined amperometric (AD). For analysis IO3–, several ...

AIM The objective of the current study is to determine the concentration of aflatoxin B1 using high-performance liquid chromatography (HPLC) with a photodiode array (PDA) detector. MATERIALS AND METHODS Aflatoxin B1 certified reference grade from Trilogy Analytical Laboratory dissolved acetonitrile (ACN) at 10 µg/mL was using standard assessment. HPLC instruments such as ultraviolet-PDA detec...

Two rapid and simple HPLC methods with UV detector to determine three main compounds (magnoflorine, spinosin and 6'''-feruloyl spinosin) and evaporative light scattering detector (ELSD) to determine jujuboside A were developed for the chemical analyses of Zizyphi Semen. Magnoflorine, spinosin, and 6'''-feruloyl spinosin were separated with an YMC J'sphere ODS-H80 column (250 mm × 4.6 mm, 4 μm) ...