The direct F.A. Method has been used for diffe­rentiation of virulent and avirulent lines of influenza viruses. A positive fluorescence was obtained indi­cating the level of virus in the tissues, sorbing with dried mouse powder helped to reduce non-specific staining. The virulent line showed the greatest num­ber of fluorescent cells with the peak at 10 hours. the non-virulent lines showed a gra...

background: approximately 600,000 deaths occur every year as a result of the acute and chronic consequences of hepatitis b virus infection. ten different hepatitis b virus genotypes have been identified with distinct geographical distributions. different clinical outcomes, including the rate of mutations, development of hepatocellular carcinoma, chronicity, response to treatment, transplantatio...

background and objectives: the obligate intracellular bacterium chlamydia trachomatis causes sexually transmissible diseases in human. timely and sensitive detection of this pathogen is very important. there are many cross-reactions in bacteriological and serological methods in detection of this type of pathogens. the aim of this study was to achieve a more specific antigen for serological test...

experimental infection of muscovy ducks with v4 strain of newcastle disease virus was undertaken to determine the response of the ducks to the virus and the possibility of virus transmission to ducks and chickens in village like conditions. twelve ducks were randomly and equally divided into three groups of control, inoculated and in-contact. additionally, the chickens were placed into two grou...

viral infections are the cause of great economic losses in the poultry industry. development of appropriate reagents and serological diagnostic kits will help to control these infections. the aim of this study is to prepare a peroxidase labeled anti-chicken igg using a mab (5b8) against chicken igg, for detection of chicken antibodies in elisa. hybridoma cells producing the mab 5b8 were culture...

bovine viral diarrhoea virus (bvdv) is an important pathogen of dairy cattle. in this study, bulk milk samples representing a total of 4105 milking cows, from 18 dairy cattle herds in the suburb of mashhad- iran, were tested for presence of bvdv by the use of a nested reverse transcription polymerase chain reaction (nested rt- pcr) assay. non of the cows in the herds had been vaccinated against...

Torque teno virus (TTV) is prevalent worldwide and has been extensively studied in human and some wild and domestic animals. As the studies on TTV in chickens was rare and there was no information about the infection of domestic village chickens with TTV and also structural resemblance of this virus to chicken anemia virus, the frequency of the infection in domestic village chickens in differen...

Since the first computer virus has been found, scanning detection has been used as a primarily method in virus detection systems. As computer viruses and worms become more complex and sophisticated, the scanning detection method is no longer able to detect various forms of viruses and worms effectively. Many anti-virus researchers proposed various detection methods including artificial immune s...