Animal gastrointestinal tract contains a complex community of microbes, whose composition ultimately reflects the co-evolution of microorganisms with their animal host. The gut microbial community of humans and animals has received significant attention from researchers because of its association with health and disease. The application of metagenomics technology enables researchers to study no...

The aim of this study was to evaluate in vitro the duration of the antimicrobial effect of endodontic sealers by means of the Direct Contact Test. The sealers tested were: Endomethasone - Septodont, Endomethasone C-Septodont, Endion-Voco, Diaket-ESPE, Pulp Canal Sealer-SybronEndo, and AH26-Dentsply DeTrey. The endodontopathic microorganisms (MO) confronted were: Staphylococcus aureus (Sa), Cand...

Rhodococcus FMF is a gram-positive bacterium isolated for the first time from soil samples of Tabriz refinery in Iran. This microorganism is able to catabolize dibenzothiophene to 2-hydroxybiphenyl and inorganic sulfur without the destruction of carbon-carbon bonds. Three structural genes, dszA, dszB, and dszC have been characterized and shown to be responsible for this phenotype. In this work,...

A facile and efficient one-pot, single-step method for deracemizing a broad range of 2-hydroxyacids to (R)-2-hydroxyacids was established by combination of resting cells of an (S)-hydroxyacid dehydrogenase-producing microorganism and an (R)-ketoacid reductase-producing microorganism.

  Background and Objectives: Nosocomial infections are one of the most important worldwide health and increased patients hospital stay, therapeutic problem and mortality rate. This study was designed to determine the frequency of microorganisms isolated from tracheal tube in patients admitted to ICU Shaheed Mostafa Khomeini Hospital Tehran, Iran, from 2001-2005. Patients and Methods: In this ...

Microorganisms with large genomes are commonly the subjects of single-round partial sequencing of cDNA, generating expressed sequence tags (ESTs). Usually there is a great distance between gene discovery by EST projects and submission of amino acid sequences to public databases. We analyzed the relationship between available ESTs and protein sequences and used the sequences available in the sec...

The work of previous investigators was confirmed in that the microorganism of rat-bite fever was found to have flagella which are clearly visible by dark-field illumination and which can be stained. The best staining is obtained with alkalinized Akashi solution, which is a modification of Giemsa's solution; the vapor of osmic acid gives the best fixation. The number of demonstrable flagella see...

The use of bacteria to effect the degradation of serologically active carbohydrates was initiated by Avery and Dubos (1930; see also Dubos and Avery 1931) in studies on the capsular carbohydrate of Pneumococcus Type III; and other microorganisms capable of splitting polysaccharides of bacterial origin have since been found. The present report concerns the isolation and cultural characters of an...

Quantitatively analyzing the (population) growth of a microorganism is a crucial task in characterizing fast-evolving microbes and in studying the effect of antibiotic agents. Conventionally, the growth of a microbial batch culture is followed by direct or indirect cell enumerating measurements on manually extracted culture aliquots at different times of incubation. Although routinely practiced...