Rhodotorula strains can produce industrial valuable bioproducts. In this work, the production of carotenoids, and lipids by glutinis using different nitrogen sources was evaluated. Two statistical experimental designs were applied to improve carotenoid production: a first 25−1 fractional factorial design evaluating influence independent variables pH, source, glucose, KH2PO4 MgSO4 concentration;...

The culture medium in many fermentations is a non-Newtonian fluid. In bacterial alginate batch production, the broth becomes more pseudoplastic as concentration increases, which impairs mixing process. This work characterizes effect of interaction between changing rheology and impeller on bioreactor fluid dynamics. Experimentally, fermentation with evolving reproduced. Three stages are mimicked...

Staphylococcus aureus (S. aureus), which has developed multidrug resistance, leads to many healthcare-associated infections resulting in significant medical and economic losses. Therefore, the development of new efficient strategies deal with these bacteria been gaining importance. Lysostaphin is a peptidoglycan hydrolase that considerable potential as bacteriocin. However, there have few repor...

In aerobic bioprocesses, oxygen is a key substrate; due to its low solubility in broths (aqueous solutions), a continuous supply is needed. The oxygen transfer rate (OTR) must be known, and if possible predicted to achieve an optimum design operation and scale-up of bioreactors. Many studies have been conducted to enhance the efficiency of oxygen transfer. The dissolved oxygen concentration in ...

Bioreactors provide suitable conditions for the growth of cells and production secondary metabolites by regulating physical chemical factors. In this study, saffron cell crocin metabolite were evaluated in Erlenmeyer flask a stirred bioreactor. To optimize conditions, effect different concentrations 2-(N-morpholino) ethanesulfonic acid (MES) (0, 2.5 5 mM) as buffering agent sucrose (3%, 6% grad...

Due to the steadily rising number of students and simultaneous reduction in practical lessons study programs, need for alternative learning resources is increasing. In this work, a method described which allows real laboratory equipment be represented as web-based models, using stirred bioreactor an example. The model that was developed explore design operation bioreactors independently time, l...

Background Enhancing throughput of bioprocess development has become increasingly important to rapidly screen and optimize cell culture process parameters. With increasing timeline pressures to get therapeutic candidates into the clinic, resource intensive approaches such as the use of shake flasks and bench-top bioreactors may limit the design space for experimentation to yield highly producti...

The feasibility of second-generation (2G) processes still highly depends on improvements in the hydrolysis and fermentation steps, ensuring whole use biomass sugars an integrated approach. main bottlenecks are differences between optimal conditions for both limitations high solid load (HSL) hydrolysis, hexoses pentose co-fermentation. To overcome these problems, present work explored a feeding ...

Miniature parallel bioreactors are becoming increasingly important as tools to facilitate rapid bioprocess design. Once the most promising strain and culture conditions have been identified a suitable scale-up basis needs to be established in order that the cell growth rates and product yields achieved in small scale optimization studies are maintained at larger scales. Recently we have reporte...