G-banding of chromosome metaphase preparations derived from haemic cells of healthy individuals and from patients with acute myeloid leukaemia was performed with the aid of trypsin, papain, and pretreatment of the chromosome spreads with emulphogene before proteolytic digestion. Papain digestion revealed more distinguishable bands than did trypsin digestion. Pretreatment of the chromosome sprea...

Abstract The objective of this study was to adapt existing in vitro methodologies determine the extent intestinal digestion corn oil (CO), canola (CA), and beef tallow (BT) via manipulation incubation length concentrations lipase, bile, calcium within a buffer solution. Unless otherwise stated, 0.5 g each lipid source were incubated separately triplicate, with triplicate batch culture runs for ...

High pressure and temperature were combined with the convenient mixture of acids to perform an effective digestion of samples from aquatic animals for phosphorus (P) analysis. Our results indicate that digestion in high-pressure bombs combined with concentrated acidic media can be a convenient way to yield all P stored in samples, and to produce accurate recoveries. Phosphorus recovery of a cer...

enzymatic extract preparation from pseudomonas aeruginosa km110 under accession no. hq730879 with lipase activity (0.3 u/ml), was used to perform enzymatic hydrolysis pretreatment of a synthetic dairy wastewater with 1000 mg/l total fat content. the pretreatment was optimized for 48 h hydrolysis time, at 45æ% c with 10% v/v enzymatic extract. the biological treatment of synthetic dairy wastewat...

Freeze-fracture cytochemistry allows visualization of cellular and molecular characteristics of biomembranes in situ. In this review, we discuss freeze-fracture cytochemistry with special reference to a new cytochemical labeling of replicas, the detergent-digestion fracture-labeling technique. In this procedure, unfixed cells are rapidly-frozen, freeze-fractured, and physically stabilized by ev...

The polymerase chain reaction (PCR) is the most widely used technique for the study of DNA. Applications for PCR have been extended significantly by the development of "long" PCR, a technique that makes it possible to amplify DNA fragments up to 40 kb in length. This article describes two novel applications of the long PCR technique, one which simplifies restriction mapping and another which en...

The ubiquity of microplastics (MPs) has now been demonstrated throughout Earth's geographic areas in both terrestrial and marine environments. Marine ecosystems are the end point plastic waste cycle, sediments increasingly considered to be a sink for debris, with possible deleterious effects on seafloor ecosystems. However, low concentration MPs complex matrix such as sediment makes their relia...

Primary adipocyte isolation by collagenase digestion is a widely used technique to study metabolic regulation and insulin action in adipocytes. However, induction of a proinflammatory response characterized by enhanced secretion of interleukin (IL)-6 has been tightly linked to the isolation process itself. To test the hypothesis that the shaking mechanical force exerted on adipocytes stimulates...