نتایج جستجو برای: slow freeze

تعداد نتایج: 125425  

Journal: :Analytical chemistry 2003
Yu Lin Gary J Gerfen Denis L Rousseau Syun-Ru Yeh

The freeze-quenching technique is extremely useful for trapping meta-stable intermediates populated during fast chemical or biochemical reactions. The application of this technique, however, is limited by the long mixing time of conventional solution mixers and the slow freezing time of cryogenic fluids. To overcome these problems, we have designed and tested a novel microfluidic silicon mixer ...

Journal: :Microscopy 2016
Masashi Yamaguchi Hiroyuki Yamada Kimitaka Higuchi Yuta Yamamoto Shigeo Arai Kazuyoshi Murata Yuko Mori Hiromitsu Furukawa Mohammad Shorif Uddin Hiroji Chibana

Structome analysis is a useful tool for identification of unknown microorganisms that cannot be cultured. In 2012, we discovered a unique deep-sea microorganism with a cell structure intermediate between those of prokaryotes and eukaryotes and described its features using freeze-substitution electron microscopy and structome analysis (quantitative and three-dimensional structural analysis of a ...

2018
Jacqueline Horn Wolfgang Friess

The collapse temperature (Tc) and the glass transition temperature of freeze-concentrated solutions (Tg') as well as the crystallization behavior of excipients are important physicochemical characteristics which guide the cycle development in freeze-drying. The most frequently used methods to determine these values are differential scanning calorimetry (DSC) and freeze-drying microscopy (FDM). ...

Journal: :Human reproduction 2007
S A Nottola G Macchiarelli G Coticchio S Bianchi S Cecconi L De Santis G Scaravelli C Flamigni A Borini

BACKGROUND We studied the ultrastructural characteristics of human mature oocytes frozen/thawed (F/T) using different concentrations of sucrose. Fresh human mature oocytes were used as controls. METHODS The oocytes (n = 48) were fixed in 1.5% glutaraldehyde at sampling (n = 16) or after freeze/thawing performed using a slow cooling method with propane-1,2-diol 1.5 mol/l and sucrose at either ...

Journal: :Human reproduction 2001
J Bischof W Fahssi D Smith T Nagel D Swanlund

Cellular level freeze injury was investigated after controlled freezing of an Eker rat uterine fibroid cell line in both the presence and absence of oestradiol. The connection between thermal history and cell injury in single ELT-3 cells in suspension (without oestradiol) was studied through a two-level, four-parameter (2(4)) experiment with membrane dye exclusion as the end-point. The four par...

M. Rabbani Khorasgani , S.H. Zarkesh-Esfahani, Sh. Sotudeh, Z. Etemadifar,

Background: Nowadays, colostrum has been known as a considerable and valuable by-product of large-scale dairy production in the world. The main objective of this study was to evaluate the effects of pasteurization, spray-drying and freeze-drying methods on bacterial loads and Immunoglobulin G (IgG) level of bovine colostrums. Methods: Colostrum samples were collected from the first milking pos...

Journal: :Human Reproduction 1997

Journal: :Journal of the Sedimentological Society of Japan 1998

Journal: :Human reproduction 2005
Xiao-Hong Li Shee-Uan Chen Xiao Zhang Miao Tang Yuan-Rong Kui Xueqing Wu Song Wang Ying-Lu Guo

BACKGROUND Surplus oocytes in assisted reproduction treatment cycles could be saved and donated to other couples. ICSI is usually performed for oocytes that have been stored frozen, considering possible exocytosis of cortical granules (CG). The unavoidability of ICSI merits further study. METHODS We used a slow method to freeze excess oocytes from infertile couples. After thawing, oocytes wer...

Journal: :iranian journal of pharmaceutical research 0
reza masaeli dental biomaterials department, school of dentistry, tehran university of medical sciences, tehran, iran teherh sadat jafarzadeh kashi dental biomaterials department, school of dentistry, tehran university of medical sciences, tehran, iran rasoul dinarvand nanotechnology research centre, faculty of pharmacy, tehran university of medical sciences, tehran 1417614411, iran mohammadreza tahriri dental biomaterials department, school of dentistry, tehran university of medical sciences, tehran, iran vahid rakhshan department of anatomy, dental branch, islamic azad univesity mehdi esfandyari-manesh nanotechnology research center,tehran university of medical sciences, tehran, iran

microspheres formulated from poly (d,l-lactic-co-glycolide) (plga), a biodegradable polymer, have been extensively evaluated as a drug delivery system. in this study, the preparation, characterization and drug release properties of the plga microspheres were evaluated. simvastatin (sim)-loaded plga microspheres were prepared by oil-in-water emulsion/solvent evaporation method. the microspheres ...

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