The review is devoted to mechanically sensitive indoline spiropyrans and their structural features. Recent advances in the study of isomerization processes are considered, special attention paid effect pressure on behavior composition various photochromic materials dyes, as well process formation J-aggregates Langmuir–Blodgett films.

Cortical dynamics can be imaged at high spatiotemporal resolution with voltage-sensitive dyes (VSDs) and calcium-sensitive dyes (CaSDs). We combined these two imaging techniques using epifluorescence optics together with whole cell recordings to measure the spatiotemporal dynamics of activity in the mouse somatosensory barrel cortex in vitro and in the supragranular layers in vivo. The two opti...

Voltage-sensitive dyes (VSDs) and optical imaging are useful for studying spatiotemporal patterns of population neuronal activity in cortical tissue. Using a photodiode array and absorption dyes we were able to detect neuronal activity in single trials before it could be detected by local field potential (LFP) recordings. Simultaneous electrical and optical recordings from the same tissue also ...

Owing to the toxicity of mercury in the environment, the determination of trace amounts of mercury has attracted great attention. I 32 The conventional technique of dithizone extraction is not always favoured because the procedure is cumbersome, sensitive to light and is subject to interferences from other metal ions.3 Analytical methods based on ternary complex formation seem to offer superior...

Bonnot, Agnès, George Z. Mentis, Jesse Skoch, and Michael J. O’Donovan. Electroporation loading of calcium-sensitive dyes into the CNS. J Neurophysiol 93: 1793–1808, 2005. First published October 27, 2004; doi:10.1152/jn.00923.2004. Calcium imaging of neural network function has been limited by the extent of tissue labeled or the time taken for labeling. We now describe the use of electroporati...

Overexpression of the antiapoptotic Bcl-2 protein enhances the uptake of fluorimetric dyes sensitive to mitochondrial membrane potential, suggesting that Bcl-2 changes the mitochondrial proton gradient. In this study, we performed calibrated measurements of mitochondrial respiration, membrane potential, deltapH, and intramitochondrial [K+] in digitonin-permeabilized PC12 and GT1-7 neural cells ...

Cy3 and Cy5 are among the most commonly used oligonucleotide labeling molecules. Studies of nucleic acid structure and dynamics use these dyes, and they are ubiquitous in microarray experiments. They are sensitive to their environment and have higher quantum yield when bound to DNA. The fluorescent intensity of terminal cyanine dyes is also known to be significantly dependent on the base sequen...

Berger T, Borgdorff A, Crochet S, Neubauer FB, Lefort S, Fauvet B, Ferezou I, Carleton A, Lüscher H-R, Petersen CC. Combined voltage and calcium epifluorescence imaging in vitro and in vivo reveals subthreshold and suprathreshold dynamics of mouse barrel cortex. J Neurophysiol 97: 3751–3762, 2007. First published March 14, 2007; doi:10.1152/jn.01178.2006. Cortical dynamics can be imaged at high...