ii Dedicated to my parents and teachers… iii i. Acknowledgements First of all, thanks to Prof. Sandro Ghisla, for providing me the opportunity to work in his lab. I am lucky indeed to have had such a friendly and lively person as my PhD supervisor. Prof. Peter Macheroux for his constructive criticisms and suggestions during this Ph.D. work and for critical reading of the manuscripts. Prof. Elis...

Chelating agents’ application for EOR and well stimulation is fast growing nowadays. However, reagents of this class have some drawbacks, with high values interfacial tension (IFT) being among them. IFT may be lowered the addition surfactants; however, simultaneous chelating agents surfactants has not yet been widely studied. This paper focuses on experimental theoretical investigation interact...

Thermostable β-galactosidases from thermophilic bacteria have attracted increasing interest to various advantages in industrial and biotechnological applications. In this study, a highly thermally stable β-galactosidase produced by Anoxybacillus sp. AH1was purified characterized. The highest enzyme production was achieved after the bacterium incubated for 24 hours. precipitation with ammonium s...

Tag der mündlichen Prüfung am: The voyage of discovery is not in seeking new landscapes but in having new eyes-Marcel Proust ABBREVIATIONS 6 Abbreviations ADAM 1-aminoadamantam ADP Adenosine diphosphate ATP Adenosine triphosphate AHT Anhydrotetracycline Amp r Resistance towards ampicilin aphA3, Kan r Resistance towards kanamycin APS Ammoniumperoxodisulfate bp Base pairs BCA Bicinchoninic acid c...

Analysis of human platelet membrane proteins by crossed immunoelectrophoresis (CIE) in the presence of Triton X-100 (TX-100) has previously shown that glycoproteins (GP) IIb and IIIa are located in a single immunoprecipitate, band 16.2 To investigate whether IIb and IIIa are associated in a complex, we have analyzed TX-100-solubilized 125I-labeled membrane proteins by density gradient ultracent...

The slow swimming mutant (strain 11C 104), isolated from Paramecium caudatum, which swims at a speed about 70% of the wild type, is not different from the wild type (strain Kyky-1) in cell shape, density of cilia over the cell surface, and length of cilia. Cross-sectioned structures of the somatic cilia also do not give any difference between the mutant and the wild type. ATPase activities of c...

Rabbit alveolar macrophages express a plasma-membrane receptor that recognizes glycoprotein ligands bearing terminal mannose, fucose or N-acetylglucosamine residues. Macrophage membranes were washed extensively with buffers containing high salt and mannose or EDTA to remove endogenously bound ligand, before Triton X-100 extraction. The extracts were chromatographed on mannose-Sepharose. Elution...