Degradation of plant N in the rumen results in the formation of ammonia-N and NAN. Rumen bacteria utilise these breakdown products to synthesise their cell proteins. This study was carried out to investigate the in vivo degradation of N-labelled duckweed in the rumen and the utilisation of the degradation products by rumen bacteria. We found that duckweed protein was relatively resistant to rum...

I. Six closely shorn sheep were given brome grass (Bromus inermis) pellets at the rate of 59 or 98 g dry matter (DM)/h and maintained at ambient temperatures of 2-5' and 22-25' for 35 d. Measurements of digestion, rate of passage of digesta, and nitrogen transformations were made during the last 13 d of temperature exposure. 2. Cold exposure at the lower level of intake reduced the apparent dig...

The objectives were to compare the ability of various rumen microbial fractions to reduce nitrate and to assess the effect of nitrate on in vitro fermentation characteristics. Physical and chemical methods were used to differentiate the rumen microbial population into the following fractions: whole rumen fluid (WRF), protozoa (Pr), bacteria (Ba), and fungi (Fu). The three nitrogen substrate tre...

nine multiparous lactating cows averaging 171 days in milk were divided according to days in milk and milk production into three 3×3 latin squares with three 3-week periods to investigate the effect of rumen degradable protein on milk urea nitrogen. diets were formulated to provide 3 concentrations of dietary rumen degradable protein (9.8, 10.8, and 11.8% of dry matter), while rumen undegradabl...

Nineteen tanniferous browse plants were collected from South Africa to investigate their digestibility, gas production (GP) characteristics and methane production. Fresh samples were collected, dried in forced oven, and ground and analyzed for nutrient composition. In vitro GP and in vitro organic matter digestibility (IVOMD) were determined using rumen fluid collected, strained and anaerobical...

Rumen microorganisms rapidly metabolized glycine at rates varying from 0.014 to 0.241 mumole of glycine per ml per min. The main metabolic products were carbon dioxide, acetic acid, and ammonia; little glycine was incorporated into bacterial protein. Use of carboxyl or methylene-labeled glycine showed that the carbon dioxide came mainly from the carboxyl of glycine, whereas both carbons of acet...

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An experiment was conducted to determine the relationship between nitrogen intake and testicular urea content. Twenty ram lambs of fat-tailed Iranian native breed were used in this study. The mean (±SD) age and body weight of the lambs were 180±20 days and 36.7±6.3 kg, respectively. The lambs were divided in to five groups of equal size, and received a ration containing a single protein source;...

Two experiments using six rumen-fistulated Holstein steers in 3 X 3 Latin square designs were conducted to test the effects of urea addition to corn silage, and grain addition to haylage, on feed intake, digestibility of nutrients and rumen fermentation of complete diets fed once daily. Addition of .34 and .68% urea to corn silage increased dietary crude protein from 8.1 to 11.3 and 13.3%, and ...

Two groups of Merino sheep (with or without rumen fistulae) were used to study the effect of feeding maize-silage (compared to hay) on: thiamine concentrations in rumen fluid, tissues and organ; blood transketolase activity; microbial fermentation in the rumen. After sheep were transferred from a hay diet to maize-silage, thiamine concentrations in ruminal fluid fell to about 25% of control val...