Mitochondrial dysfunction is a key driver of neurodegeneration. This study aimed to evaluate the protective potential EPOR/CD131 (heterodimeric erythropoietin receptor) stimulation in neurodegeneration caused by rotenone-induced mitochondrial dysfunction. The effects (EPO) and an EPO mimetic peptide pHBSP were assessed using vivo vitro models. Single injections 10 µg/kg or 5 significantly allev...

In September 2006, Diamond Lake (OR, USA) was treated by the Oregon Department of Fish and Wildlife with a mixture of powdered and liquid rotenone in the successful eradication of invasive tui chub Gila bicolor. During treatment, the lake was in the middle of a phytoplankton (including cyanobacteria Anabaena sp.) bloom, resulting in an elevated pH of 9.7. Dissipation of rotenone and its major m...

The effect of semicarbazide on the oxidation of choline by rat liver mitochondria was studied. Semicarbazide was used to prevent both further oxidation of betainealdehyde and product inhibition of choline dehydrogenase by the aldehyde. NAD did not stimulate choline-based respiration when semicarbazide was present to prevent the oxidation of enzymatically formed betainealdehyde. In contrast, NAD...

As a major co-morbidity of Parkinson's disease (PD), depression is associated with the loss of serotonergic neurons. Our recent study has shown that midbrain dopaminergic neurons are particularly vulnerable to microtubule-depolymerizing agents including rotenone, an environmental toxin linked to PD. Here we show that rotenone also selectively killed serotonergic neurons in midbrain neuronal cul...

Previous studies revealed that pesticides interact with α-synuclein and accelerate the rate of fibrillation. These results are consistent with the prevailing hypothesis that the direct interaction of α-synuclein with pesticides is one of many suspected factors leading to α-synuclein fibrillation and ultimately to Parkinson's disease. In this study, the biophysical properties and fibrillation ki...

Partial inhibition of mitochondrial respiratory complex I by rotenone reproduces aspects of Parkinson's disease in rodents. The hypothesis that rotenone enhancement of neuronal cell death is attributable to oxidative stress was tested in an acute glutamate excitotoxicity model using primary cultures of rat cerebellar granule neurons. As little as 5 nM rotenone increased mitochondrial superoxide...

Tianma Gouteng Yin (TGY) is a traditional Chinese medicine (TCM) decoction widely used to treat symptoms associated with typical Parkinson's disease (PD). In this study, the neuroprotective effects of water extract of TGY were tested on rotenone-intoxicated and human α-synuclein transgenic Drosophila PD models. In addition, the neuroprotective effect of TGY was also evaluated in the human dopam...

Rotenone, a widely used pesticide and an environmental risk factor for Parkinson's disease (PD), induces nigrostriatal injury, Lewy body-like inclusions, and Parkinsonian symptoms in rat models for PD. Our previous data indicated that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) overexpression and glycolytic inhibition were co-current in rotenone-induced PC12 (rat adrenal pheochromocytoma c...

Antiviral activity on Newcastle disease virus was examined with some respiration inhibitors including ascochlorin, rotenone, antimycin A(3), piericidin A, dicoumarol, 2, 4-dinitrophenol, pentachlorophenol, and fatty acids. Of the chemicals tested, ascochlorin and rotenone showed significant inhibitory effect on the viral growth in cultured cells as determined by the plate and tube assay methods...

Mitochondrial dysfunction is associated with various forms of lung injury and disease that also involve alterations in pulmonary endothelial permeability, but the relationship, if any, between the two is not well understood. This question was addressed by perfusing isolated intact rat lung with a buffered physiological saline solution in the absence or presence of the mitochondrial complex I in...