Erythrocytes containing pig liver uricase have been prepared by hypotonic hemolysis in the presence of the enzyme. Uricase is shown to be active within the erythrocytes and to degrade uric acid as rapidly as it enters the cells when high intracellular enzyme concentrations are employed. The kinetics and characteristics of uric acid entry are shown to be the same for hemolysed and normal erythro...

336 CLINICALCHEMISTRY, Vol.25,No. 2, 1979 capillary, the nine negative specimens were sampled and processed. This experiment was repeated with a plastic capillary. With the glass capillary, the first specimen was positive as expected, but the next eight specimens were also positive. The last specimen was negative. For the plastic capillary, the first specimen was positive and the next three ori...

Two efficient procedures are presented for the purification of the purine catabolic enzyme uricase from Neurospora crassa. A specific antiserum for uricase was prepared and used to examine the regulation of uricase expression. Even when wild-type cells are growing under full nitrogen repression conditions, they possess a considerable basal level of uricase. Induction results in a severalfold in...

We describe our optimization and evaluation of a candidate Reference Method for uric acid in serum. Reaction parameters were optimized for a manual, enzymic method for uric acid in which highly purified microbial uricase is used to quantitate uric acid by a differential ultraviolet procedure. We evaluated the method in terms of freedom from interferences, analytical recovery, precision, and com...

A 62-year-old man with a complaint of back pain lasting 2 months was admitted. He also presented a huge abdominal tumor. Diagnostic imaging showed metastatic tumors in the liver, lumbar vertebrae and bilateral lung. An ultrasound-guided needle biopsy revealed a lung tumor containing melanic tissue. Subsequently, there was an evident elevation in uric acid, phosphoric acid, potassium and lactate...

A 64-year-old male presented to hospital with breathlessness and weight loss. Ultrasound-guided biopsy of supraclavicular lymph node confirmed a diagnosis of small-cell lung cancer. The patient was started on Dexamethasone 8 mg twice daily for symptom control while awaiting urgent oncology assessment. Three days later he was admitted with acute kidney injury and worsening breathlessness. Bioche...

• Hyperuricemia associated with tumor lysis syndrome is a potentially serious complication most frequently seen during chemotherapy for malignancies with large tumor burden and highly proliferative cells, such as aggressive lymphomas and leukemia.1-3 Hyperuricemia usually occurs in association with other metabolic abnormalities, including hyperphosphatemia, hyperkalemia, hypocalcemia, and azote...

In 1947 Kalckar introduced a comprehensive differential spectrophotometric system for the analysis of purines, including uric acid (1). Praetorius and his associate Poulsen employed this procedure and have published detailed directions for a practical uric acid method (2, 3). During the past 4 years, over 2000 routine uric acid determinations on blood and urine have been performed in this labor...