Linum usifafissimum protoplast-derived colonies that are cultured in auxin-supplemented medium and immobilized in Ca2+alginate matrix form round colonies that develop into polarized, embryo-like structures. On the other hand, protoplast-derived colonies that are immobilized i n agarose do not show an organized morphogenetic response, and unique, ionically bound cell-wall protein patterns match ...

Herein we are the first to report that single-walled carbon nanotubes (SWCNTs) exhibit dual-phase regulation to Arabidopsis mesophyll cells exposed to different concentration of SWCNTs. The mesophyll protoplasts were prepared by enzyme digestion, and incubated with 15, 25, 50, 100 μg/ml SWCNTs for 48 h, and then were observed by optical microscopy and transmission electron microscopy, the react...

Abiotic stresses have considerable negative impact on Mediterranean plant ecosystems and better comprehension of the genetic control of response and adaptation of trees to global changes is urgently needed. The single cell gel electrophoresis (SCGE) assay could be considered a good estimator of DNA damage in an individual eukaryotic cell. This method has been mainly employed in animal tissues, ...

Background: In Algeria, date palm is currently confronted to the Bayoud disease. Biotechnological tools such as protoplasts fusion can appear as an alternative to ensure rapid multiplication and improvement of this species. Objectives: Callogenesis induction in protoplasts isolated from embryogenic callus of three date palm cultivars. Materials and Methods: Some factors influencing the isolatio...

Abstract Roots are essential for plant growth and development. Bamboo is a large Poaceae perennial with 1,642 species worldwide. However, little known about the transcriptional atlas that underpin root cell-type differentiation. Here, we set up modified protocol protoplast preparation reported single-cell transcriptomes of 14,279 filtered single cells derived from basal tips Moso bamboo. We ide...

Linum usitatissimum protoplast-derived colonies that are cultured in auxin-supplemented medium and immobilized in Ca(2+)-alginate matrix form round colonies that develop into polarized, embryo-like structures. On the other hand, protoplast-derived colonies that are immobilized in agarose do not show an organized morphogenetic response, and unique, ionically bound cell-wall protein patterns matc...

Abstract California poppy ( Eschscholzia californica ) is a member of the Ranunculales, sister order to all other eudicots and as such in phylogenetically highly informative position. Ranunculales are known for their diverse floral morphologies biosynthesis many pharmaceutically relevant alkaloids. E. it widely used model system study conservation flower developmental control genes. However, wi...