نتایج جستجو برای: prokaryotic expression
تعداد نتایج: 879087 فیلتر نتایج به سال:
Background: Dense granule antigens (GRA antigens) of Toxoplasma gondii induce strong antibody response in humans and are considered as useful diagnostic antigens. Previous studies reported expression of amino terminal GRA8 protein in fusion with large tags such as glutathione-S-transferase. The present study aimed to produce soluble full length immunogenic GRA8 in bacteria. Methods: GRA8 comple...
Chloroplasts are semiautonomous organelles which possess their own genome and gene expression system. However, extant chloroplasts contain only limited coding information, and are dependent on a large number of nucleus-encoded proteins. During plant evolution, chloroplasts have lost most of the prokaryotic DNA-binding proteins and transcription regulators that were present in the original endos...
conclusions: our data showed that the recombinant mature lysostaphin protein produced by pet32a vector in e. coli system was very efficient. results: pcr and sequencing results confirmed the successful cloning of the target gene into the vector. the expression of protein was induced by iptg and high concentration of the recombinant protein was obtained via the purification process by affinity-c...
The paper summarizes the current status of the reporter gene technology and their basics. Reporter gene technology is widely used to monitor cellular events associated with gene expression and signal transduction. Based upon the splicing of transcriptional control elements to a variety of reporter genes, it “reports” the effects of a cascade of signaling events on gene expression inside cells. ...
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