نتایج جستجو برای: preantral follicle
تعداد نتایج: 21081 فیلتر نتایج به سال:
objective: improvements in cancer treatment have allowed more young women to survive. however, many cancer patients suffer from ovarian failure. cryopreservation is one of the solutions for fertility restoration in these patients. the cryopreservation of isolated follicles is a more attractive approach in the long term. many endocrine and paracrine factors can stimulate the granulosa cells of p...
BACKGROUND A standard histomorphometric approach has been used for nearly 40 years that identifies atretic (e.g., dying) follicles by counting the number of pyknotic granulosa cells (GC) in the largest follicle cross-section. This method holds that if one pyknotic granulosa nucleus is seen in the largest cross section of a primary follicle, or three pyknotic cells are found in a larger follicle...
BACKGROUND Effects of androgens on follicle maturation have been controversial for some time. Here, we review the potential of their applications in improving human ovulation induction, based on human and animal data, reported in the literature. METHODS We reviewed the published literature for the years 2005-2011, using relevant key words, in PubMed, Medline and Cochrane reviews, and then per...
The structure of follicular tissue affects the ability to maintain structural integrity follicles against cryoinjury post-vitrification. Histological analysis was conducted on each stage preantral post-vitrification using 7.5% and 15.0% doses ethylene glycol (EG), ovarian sections with HE staining were observed an Olympus CX21 microscope connected Optilab 3.0 lens Image Raster software. Analysi...
Encapsulated three-dimensional culture supports development of nonhuman primate secondary follicles.
In vitro ovarian follicle cultures may provide fertility-preserving options to women facing premature infertility due to cancer therapies. An encapsulated three-dimensional (3-D) culture system utilizing biomaterials to maintain cell-cell communication and support follicle development to produce a mature oocyte has been developed for the mouse. We tested whether this encapsulated 3-D system wou...
Regulation of ovarian activity is an integrated process encompassing both extraovarian signals and intrafollicular factors. Initiation of primordial follicle growth and the early stages of folliculogenesis can occur without gonadotropins, but FSH may affect the rate of preantral follicle growth. Antral follicle development from 1 to 4 mm in sheep and cattle is completely gonadotropin dependent....
A sequential medium was evaluated on the survival, activation and growth rates of caprine preantral follicles submitted to a long-term culture period, aiming to establish an ideal in vitro culture system. Ovarian fragments were cultured for 16 days in α-MEM(+) alone or supplemented with hormones (GH and/or FSH) added sequentially on different days of culture. Ovarian fragments were cultured in ...
Backgroundovarian follicle growth and oocyte maturation. In vitro follicular culture systems, at variousdevelopmental stages, allow the identification of these factors and the understanding of theirmechanisms of action. Keeping in mind the significance of the influence of environmental factors onthe follicle growth, this work focuses on the effect of some endocrine and paracrine factors on theg...
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