Polyhydroxyalkanoates (PHAs) have been demonstrated to be a family of biopolymers with good biodegradability and biocompatibility. To mimic the real microenvironment of extracellular matrix (ECM) for cell growth, novel nanofiber matrices based on PHA polymers were prepared via a phase separation process. Three-dimensional interconnected fibrous networks were observed in these matrices with aver...

Polyhydroxybutyrate (PHB) is a sustainable polymer that promising candidate for replacing petroleum-based plastics in food packaging. Fillers are used to improve the mechanical properties of PHB composites, simultaneously changing crystallinity matrix. However, it not well understood how fillers affect crystallisation and microstructure, thus resulting composite. This review summarises simulati...

382 ©2014 Society for Biomaterials

Application of polyhydroxybutyrate (PHB) to plastic industry has expanded over the last decades due to its attracting features over petro-based plastic, and therefore, its waste accumulation in nature is inevitable. In the present study, a total of four bacterial strains, viz., MK3, PN12, PW1, and Lna3, were formulated into a consortium and subsequently used as biological tool for degradation o...

The production of 1,3-propanediol (1,3-PD) from glycerol by Klebsiella pneumoniae is limited synthesis numerous byproducts. Among them, the accumulation acetate has largest negative impact on fermentation performance. To address overflow caused knocking out lactate dehydrogenase, alcohol dehydrogenase and succinate several metabolic engineering manipulations were conducted. First, was reduced t...

The ccpA gene was inactivated in the polyhydroxybutyrate (PHB)-producing strain Bacillus sp. MA3.3 in order to reduce glucose catabolite repression over pentoses and develop improved bacterial strains for the production of PHB from lignocellulosic hydrolysates. Mutant Bacillus sp. MSL7 ΔCcpA are unable to grow on glucose and ammonia as sole carbon and nitrogen sources, respectively. Supplementa...

Classical microbial carbon polymers such as glycogen and polyhydroxybutyrate (PHB) have a crucial impact as both a sink and a reserve under macronutrient stress conditions. Most microbial species exclusively synthesize and degrade either glycogen or PHB. A few bacteria such as the phototrophic model organism Synechocystis sp. PCC 6803 surprisingly produce both physico-chemically different polym...

Abstract This work aimed to study the feasibility of using vinasse for polyhydroxybutyrate (PHB) production by Bacillus megaterium . To optimize culture medium, a Box–Behnken design was employed considering carbon (C), nitrogen (N), and phosphorus (Ph) concentrations as independent variables PHB productivity response variable. The decreased when C or N were increased, probably due presence phen...

A diverse and versatile spectrum of metabolic activities among isolates of fluorescent Pseudomonas putida indicates their adaptability to various niches. These polyhydroxybutyrate producing and phosphate solubilizing isolates showed a high level of functional and genetic versatility among themselves. One of the potential P. putida isolate P132 can contribute as a candidate agent for both biocon...

The undefined poly(3-hydroxybutyrate)- (PHB-) negative mutant R. eutropha PHB-4 was generated in 1970 by 1-nitroso-3-nitro-1-methylguanidine (NMG) treatment. Although being scientific relevant, its genotype remained unknown since its isolation except a recent first investigation. In this study, the mutation causing the PHA-negative phenotype of R. eutropha PHB-4 was confirmed independently: seq...