In article number 1700578, Jianzhong Xu, Shiwu Dong, and co-workers report the development of a graphene-based miRNA transfection system that uses a polyethylenimine (PEI) functionalized graphene oxide complex loaded with miR-7b plasmid. The system efficiently delivers the miR-7b plasmid into bone marrow macrophases and reduces the expression of target protein dendritic cell-specific transmembr...

Structurally well-defined ABA triblock copolymers with low molecular weight linear polyethylenimine (PEI) as A block and poly(ethylene glycol) (PEG) as B block have been prepared and used for gene delivery. PEI–PEG–PEI 2100–3400–2100 can effectively condense DNA, giving small-sized polyplexes (b100 nm) with reduced zeta-potentials (b10 mV). Initial experiments show that these polyplexes have a ...

Reversing pathologic alterations in vascular microRNA (miRNA) expression represents a potential therapeutic strategy for pulmonary hypertension. While polyethylenimine (PEI) has previously been shown to be an effective vehicle for vascular lung-directed delivery of plasmid DNA, it remains unclear whether this utility is generalizable to miRNAs. Here we show that despite elevated lung levels, th...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 208 No. 7 881–896 www.jcb.org/cgi/doi/10.1083/jcb.201412085 JCB 881 Correspondence to Gordon K.T. Chan: [email protected] J.K. Famulski’s present address is Department of Biology, University of Kentucky, Lexington, KY 40506. Abbreviations used in this study: ACA, anticentromere antibody; CENP, centromere protein; FTI, farnesyl transferase...

N,N-dodecyl,methyl-polyethylenimine coatings applied to solid surfaces have been shown by us to disinfect aqueous solutions of influenza viruses. Herein we elucidate the mechanism of this phenomenon. Infectivity-, protein-, RNA-, and scanning electron microscopy-based experiments reveal that, upon contact with the hydrophobic polycationic coating, influenza viruses (including pathogenic human a...

objective(s) nonviral vector can be an attractive alternative to gene delivery in experimental study. in spite of some advantages in comparison with the viral vectors, there are still some limitations for efficiency of gene delivery in nonviral vectors. to determine the effective expression, the recombinant escherichia coli lacz genes were cloned into the different variants of pcdna3.1 and then...