Despite the large number of techniques available for the transformation of bacteria, several species are still resistant to the introduction of foreign DNA. Oenococcus oeni are among the organisms that are particularly refractory to transformation. However, conjugal experiments from Lactococcus lactis to O. oeni with a new plasmid, pGID052, were performed via mobilization with success. This pla...

background: serological assay based on dense granular (gra) proteins of toxoplasma gondii (t. gondii) is actually the most popular laboratory diagnostic tool to detection of toxoplasmosis. we aimed to construct a recombinant gra7-ptz57rt plasmid vectors that it is suitable for sub-cloning and gra7 protein production. m aterials and methods: souris mice were used for maintaining of t. gondii tac...