introduction: the a im of this study is the evaluation of the effect of antimicrobial photodynamic therapy with radachlorin on staphylococcus aureus and escherichia coli. new windows are open in the antimicrobial field so-call photodynamic therapy that incorporates a nonpoisonous photosensitizer (ps) with innocuous special wavelength photons to excite the ps. methods: two strains of bacteria us...

Photoimmunotherapy was introduced two decades ago but has been studied infrequently in vivo and is virtually untested clinically. Progress has been limited because high-quality, well-characterized photosensitizer immunoconjugates (PICs) have been difficult to make. Here, we describe the development of an innovative conjugation method for producing water-soluble PICs that are free of insoluble a...

PURPOSE The rate of energy delivery is a principal factor determining the biological consequences of photodynamic therapy (PDT). In contrast to conventional high-irradiance treatments, recent preclinical and clinical studies have focused on low-irradiance schemes. The objective of this study was to investigate the relationship between irradiance, photosensitizer dose, and PDT dose with regard t...

AIM OF THE STUDY Photodynamic therapy (PDT) is an approved, minimally invasive and highly selective therapeutic approach to a variety of tumors. It is based on specific photosensitizer accumulation in the tumor tissue, followed by irradiation with visible light. The photochemical interactions of the photosensitizer, light and molecular oxygen produce singlet oxygen and other reactive oxygen for...

Photodynamic therapy is emerging as a viable modality for the treatment of many cancers. A limiting factor in its use against intracavity tumors such as disseminated ovarian cancer is insufficient selectivity of the photosensitizer for tumor compared with normal tissue. We report on an approach to improve tumor targeting by exploiting differences between cell types and by chemical modification ...

To investigate the mechanism of inhibition of breast cancer cell proliferation by a newly developed photosensitizer chrolophyll derivative CPD4. MCF-7 cells were treated with CPD4 in different concentrations for 2 h, followed by irradiation (670 nm, 10 J/cm(2)) using a semiconductor laser. Apoptotic mode and ratio of treated cells were analyzed. Subcellular localization of CPD4 were observed. M...

OBJECTIVES The growing resistance of methicillin-resistant Staphylococcus aureus (MRSA) to conventional antimicrobial agents necessitates the development of alternative approaches to preventing and treating infections. One such approach is photodynamic therapy, whereby target cells are treated with light-activated drugs (photosensitizers). This investigation aimed to determine whether the abili...

The objective of this study was to evaluate the effect of photodynamic therapy with erythrosine and rose bengal using a light-emitting diode (LED) on planktonic cultures of S. mutans. Ten S. mutans strains, including nine clinical strains and one reference strain (ATCC 35688), were used. Suspensions containing 10⁶ cells/mL were prepared for each strain and were tested under different experiment...

We present a quantitative framework to model a Type II photodynamic therapy (PDT) process in the time domain in which a set of rate equations are solved to describe molecular reactions. Calculation of steady-state light distributions using a Monte Carlo method in a heterogeneous tissue phantom model demonstrates that the photon density differs significantly in a superficial tumor of only 3 mm t...