BACKGROUND Ano-genital carcinoma is a polyfactorial and polygenic disease. Certain strains of human papillomavirus (HPV) have been detected in a high percentage of patients. It has been suggested that p53 polymorphisms may be relevant for the interaction with viral proteins that inactivates p53. MATERIALS AND METHODS Patients were selected on the basis of HPV infection, clinical history, posi...

objective: helicobacter pylori is a spiral, microaerophilic gram negative bacterium, that multiplies and causes infection in human gastric mucosal layer. h.pylori infection, followed by destruction of gastric epithelial tissue, leads to gastric chronic inflammation, which can cause gastric and peptic ulcers. new approaches have focused on using specific treatments, such as immunotherapy, to era...

The aim of the present study was to evaluate anxiety and depression status lung cancer patients during coronavirus disease 2019 (COVID‑19) pandemic effects psychological counseling online. For this purpose, following enrollment. were divided into an intervention group (group I) a control C). Participants in two groups scored using Self‑Rating Anxiety Scale (SAS) Depression (SDS) before one week...

Software testing is the methodology of analyzing nature software to test if it works as anticipated so boost its reliability and quality. These two characteristics are very critical in applications present times. When testers want perform scenario evaluations, oracles generally employed third phase. Upon case execution outcome generation, essential validate results establish behavior’s correctn...

Tricine–SDS-PAGE is commonly used to separate proteins in the mass range 1–100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in other electrophoretic systems. These lower concentrations facilitate electroblotting, which is particularly crucial for hydrophobic proteins. Tricine–...

A polymerase chain reaction (PCR)-based method was developed to detect DNA of Fusarium solani f. sp. glycines , the cause of soybean sudden death syndrome. Two pairs of primers, Fsg1/Fsg2 designed from the mitochondrial small subunit ribosomal RNA gene, and FsgEF1/FsgEF2 designed from the translation elongation factor 1α gene, produced PCR products of 438 and 237 bp, respectively. Primer specif...

Mbofung, G. C. Y., Fessehaie, A., Bhattacharyya, M. K., and Leandro, L. F. S. 2011. A new TaqMan real-time polymerase chain reaction assay for quantification of Fusarium virguliforme in soil. Plant Dis. 95:1420-1426. The quantification of the soilborne pathogen Fusarium virguliforme inoculum in soil is important for epidemiological studies of soybean sudden death syndrome (SDS). Classical dilut...

OBJECTIVES The aim of this study was to evaluate the presence of carbapenemases in a Klebsiella pneumoniae collection and the performance of the modified Hodge test (MHT) to correctly identify this phenotype. METHODS Twenty-eight K. pneumoniae clinical isolates with reduced susceptibility to carbapenems were evaluated. Antimicrobial susceptibility and molecular typing were performed by agar d...