BACKGROUND The purpose of this study was to investigate the chromosomal complement and developmental potential of in-vitro matured murine oocytes following ICSI by human sperm. METHODS Heterologous ICSI fertilization between mouse oocytes and human sperm was employed in order to overcome the reduced fertilization rates observed after conventional IVF due to zona hardening during in-vitro matu...

Background We have previously reported the effect of Anthocyanin and cAMP modulators on maturation and apoptosis of sheep oocytes. In this study, we investigated the reduction capacity and apoptotic gene expression results at MII sheep oocytes. MaterialsAndMethods Good ovine Cumulus-oocyte complexes (COCs) were transferred to Pre-IVM medium, then to two type IVM mediums: 1) 0.1μg/ml Delphinidin...

BACKGROUND Birefringent spindles imaged with the Polscope can predict fertilization rates after intracytoplasmic sperm injection (ICSI). The present study examined the development of human oocytes with or without birefringent spindles, imaged with the Polscope before ICSI. METHODS Oocytes were obtained from stimulated ovaries of consenting patients undergoing oocyte retrieval for ICSI. Spindl...

objective: phthalates, which are commonly used to render plastics into soft and flexible materials, have also been determined as developmental and reproductive toxicants in human and animals. the purpose of this study was to evaluate the effect of mono-(2- ethylhexyl) phthalate (mehp) and di-(2-ethylhexyl) phthalate (dehp) oral administrations on maturation of mouse oocytes, apoptosis and gene ...

This study was designed to compare the effectiveness of different activation treatments for activation of in vitro matured oocytes and their developmental potency in mCR2aa medium so as to obtain maximum number of embryos. A total of 1090 cumulus oocyte complexes (COC’s) were collected from 480 ovaries. In vitro matured oocytes were randomly divided into four groups. Group 1 in vitro matured oo...

Abstract Background Estradiol is an important marker of ovarian response to stimulation in ART cycles. The study tries find the correlation serum estradiol on day HCG trigger number follicles, oocytes retrieved, and mature oocytes, also, correlate per follicle oocyte HCG, oocytes. It a cross sectional using retrospective data. Results data 232 patients were analyzed. Our showed positive between...

This study was designed to investigate the effect of addition several concentrations royal jelly media used in vitro maturation process Iraqi sheep. The ovary brought from Al-Shulla slaughterhouse and transferred obstetric laboratory at College Veterinary Medicine University Baghdad by a thermal insulating box containing physiological solution with antibiotic penicillin temperature 30-38°C. In ...

Cryopreservation of matured oocytes is a useful technique because the oocytes can be used for some assisted reproductive technologies after warming. Even though rats, like mice, have been used in various research fields including reproductive technology, information about cryopreservation of rat oocytes is limited. The objective of the present study was to improve the vitrification protocol for...