In this paper, a method for three-dimensional modeling of tea-shoots with images and calculation models is introduced. The process is as follows: the tea shoots are photographed with a camera, color space conversion is conducted, using an improved algorithm that is based on color and regional growth to divide the tea shoots in the images, and the edges of the tea shoots extracted with the help ...

A plant regeneration protocol was devised for Cardiospermum halicacabum by means of aseptically extracted 7 days old hypocotyls forming adventitious shoots on Murashige and Skoog (MS) medium harmonized with 0.7 μM thidiazuron (TDZ) producing a maximum of 18.20 ± 0.98 number of shoots in 94% cultures following 4 weeks. Subsequent subculturing for five passages, on a medium without plant growth r...

Highly efficient in vitro regeneration system has been developed for Swertia lawii Burkill, an important herb used as substitute for Swertia chirayita. Shoot tips explants were cultured on MS medium with various phytohormones for multiple shoot production. The best shoot production frequency (100%) and maximum shoots (10.4 ± 0.8) were obtained on MS media containing TDZ (3.0 mg l-1) in combinat...

In vitro propagation of rose (Rosa indica) as well as rose breeding is a valuable economic enterprise and has received highest importance globally including Indian subcontinent. The development of new cultivars for commerce is faced with new challenges. In vitro micropropagation protocols of varieties of roses have been established but very few reports on the clonal propagation of local cultiva...

Biological control of Canada thistle using Puccinia punctiformis has been largely unsuccessful in part due to a low incidence of systemically infected shoots and heterogeneous distribution of teliospores in the soil. The present study investigated the feasibility of strategic mowing to improve incidence of systemically infected shoots, and enhance intraand/or inter-season disease development in...

Indole-3-acetyl-myo-inositol esters have been demonstrated to be endogenous components of etiolated Zea mays shoots tissue. This was accomplished by comparison of the putative compounds with authentic, synthetic esters. The properties compared were liquid and gas-liquid chromatographic retention times and the 70-ev mass spectral fragmentation pattern of the pentaacetyl derivative. The amount...

The properties of homoserine dehydrogenase (EC 1.1.1.3) isolated from shoots of young etiolated seedlings of Zea mays L. var. earliking can be reversibly altered by dialysis against an appropriate buffer. Treatment with 500 millimolar potassium phosphate buffer (pH 7.5) in the absence of l-threonine results in diminished regulatory control such that the enzyme becomes less sensitive to feedback...

Toona sinensis Roem is a popular leafy vegetable in Chinese cuisine and is also used as a traditional Chinese medicine. In this study, leaf samples were collected from the same plant on two development stages and then used for high-throughput Illumina RNA-sequencing (RNA-Seq). 125,884 transcripts and 54,628 unigenes were obtained through de novo assembly. A total of 25,570 could be annotated wi...

In vitro plantlet regeneration has been obtained from 20-25days old seedling shoot tip segments of Cucumis melo (L.). We were examined using various phyto hormones individually and in combination on Murashige and Skoog (MS) semi solid medium supplemented with BAP (1.0-3.0 mg/L) Kn (1.0-3.0 mg/L),IAA (0.5 mg/L)+ BAP (1.0-3.0 mg/L) and IAA (0.5 mg/L)+Kn (1.0-3.0 mg/L) for multiple shoots prolifer...