Incorporation of [nH]threonine and [3H]glucose by the mucous and serous cells of the human bronchial submucosal gland has been studied over 8 h using, for the first time, in vitro pulse labeling and electron microscope autoradiography. In assessing the autoradiographs, two methods were compared, the circle analysis and the recently described hypothetical grain analysis. Preliminary studies show...

Fungal pores feel the pull S teinberg et al. report that fungi use motor proteins to distribute their nuclear pores around the nuclear envelope. In animal cells, the nuclear lamina holds the nuclear pores in place, ensuring that they are evenly spaced. The lamina prevents nuclear pores from clustering, promoting import of cargoes through the pores and helping to organize the chromosomes, which ...

The mechanism of the formation of alveolar pores (Kohn's pores) in neonatal male rabbits was studied by scanning electron microscopy and scanning electron microscopic morphometric analysis. The number of alveolar pores per alveolus was increased after birth. About 80 percent f alveolar pores had Type H alveolar epithelial cells on their edges when the rabbits were I-day old. This percentage dec...

Some 30 lectins in combination with glycosidase digestion and immunohistochemistry with 5 antibodies directed against antigens of the ABO and Lewis blood group systems were used to analyse the distribution and synthesis of glycoconjugates in the epithelium of the large airways in man. Both mucous gland cells and goblet cells were labelled by 12 of 30 lectins and by the antibodies, dependent on ...

Exposure of pulmonary airways to environmental toxins and allergens may cause proliferation of airway epithelial cells and mucous cell metaplasia (MCM); however, it is unclear to what extent proliferating cells differentiate into mucus-storing cells and contribute to MCM. Our previous studies demonstrated that Bcl-2, an inhibitor of apoptosis with cell cycle regulatory functions, is expressed i...

Retinoid-deficient cultures of airway epithelial cells undergo squamous differentiation. Treatment of such cultures with retinoic acid (RA) leads to restoration of the mucous phenotype. The purpose of our study was to characterize the cellular and molecular changes following RA treatment of retinoid-deficient human tracheobronchial epithelial cell cultures. Of particular interest was to determi...