abstract gerbera is one of the most demanded plants in commercial cut flowers plants. optimizing the micro-propagation of new cultivars is therefore important. in this research “red explosion” cultivar was used and capitola (as explants) were directed to a modified solid murashigue and skoog medium with four concentration of tdz (0.25, 0.50, 0.75 and 1.00 mgl-1) for regeneration. three concentr...

background: progress achieved in culture media formulations have resulted led to an improvement in maintaining the mammalian embryo in culture throughout the preimplantation and pre-attachment period. objective: the objective of this study was to evaluate the effect of various concentrations of myo-inositol during in vitro fertilization of bovine oocytes on subsequent embryo development. materi...

An axenic culture o f Symphyogyna brongniartii has been initiated from spores on modified Benecke medium. Growth was best on Gamborg B5 medium with 0.3% sucrose, with a doubling time o f 36 days. The culture produced the same terpenoids as plants growing in their natural habitat, perrottetianal A being the major constituent. Also found were bicyclogermacrene, ß-barbatene, 8-selinene, ß-cubebene...

One-cell CF-1 x B6SJLF1/J embryos, which usually exhibit a 2-cell block to development in vitro, have been cultured to the blastocyst stage using CZB medium and a glucose washing procedure. CZB medium is a further modification of modified BMOC-2 containing an increased lactate/pyruvate ratio of 116, 1 mM-glutamine and 0.1 mM-EDTA but lacking glucose. Continuous culture of one-cell embryos in CZ...

introduction: hela cell is a widely studied cell line derived from human cerzix adenocarcinoma. the cells are epithelial - like in morphology and susceptible to polio viruses and adenovirus type 5. hela cells are usually as monolayer for isolation and propagation of some human viruses, as well as for the expression of recombinant proteins, including hepatitis b surface antigen. the aim of this ...