Mass vaccination against viral infections such as mumps is a very noticeable and appropriate attempt in common health. Mumps infection has severe complications like deafness, infertility and meningitis. Attenuated live vaccine of mumps is produced and used to prevent the problems. Viral content in the monovalent vaccine is assayed with different methods. Nowadays international standards intro...

Comparison between Microplate Spectrometry and LC/MS Chromato-graphy for Facile Pilot Pharmacokinetics Biodistribution Studies of Doxorubicin-loaded Nanoparticle Drug CarriersPengxiao Cao Younsoo Bae

Optical density (OD) measurements of microbial growth are one of the most common techniques used in microbiology, with applications ranging from studies of antibiotic efficacy to investigations of growth under different nutritional or stress environments, to characterization of different mutant strains, including those harbouring synthetic circuits. OD measurements are performed under the assum...

On October 9, 2014, a Mw 7.1–6.7 seismic doublet occurred at the Juan Fernández microplate, close to triple junction with Pacific and Nazca plates. The 7.1 earthquake is largest ever have been recorded in region. Its thrust focal mechanism also unusual for region, although northern part of microplate expected undergo compression. region remote seismological data limited station ~600 km distance...

ABSTRACT The dynamics of the acoustic streaming and the acoustic positioning performance in an ultrasound-actuated multiwell microplate are investigated by two different ultrasonic frequency actuation schemes: Frequency-modulation and single-frequency actuation. Our results show a significant decrease in size of the field of view when using frequencymodulation compared to single-frequency actua...

The effectiveness of microwave-assisted protein digestion in different well positions of a 96-well microplate was investigated where microwave-assisted protein digestion of bovine serum albumin was performed in 10 different wells of a 96-well microplate in a microwave oven. Similarly increased sequence coverages (~70%) were generally observed for the 10 microwaveassisted protein digestion sampl...

Our publishing company MDPI AG has its headquarters in Basel, Switzerland where there are thousands of scientists working in the laboratories of pharmaceutical companies and institutes including Novartis [1], F. Hoffmann-La Roche [2] and institutes affiliated with University of Basel [3]. In 1996, the first annual microplate conference MipTec was held in Basel, and the MipTec 2011 was held a fe...

We describe microplate methods for measurement of human urinary albumin (HUA) by competitive enzyme-linked immunosorbant assay (ELISA) and creatinine with a modified commercial enzymatic kit. Incorporation of substrate mixing into the competitive ELISA changes the dynamic absorbance-concentration response, greatly simplifying calculations and improving sensitivity and accuracy. Measurement of c...

A fast and simple assay for T7 RNA polymerase based upon chemiluminescent detection of the synthesized, digoxigenin-labeled RNA on a nylon membrane with anti-digoxigenin coupled alkaline phosphatase and CSPD as substrate is described. Activity of RNA polymerase is determined with high sensitivity by quantifying the emitted light of the microplate-formatted dot-blot membrane with a photon counti...