Quantitative biology relies on the construction of accurate mathematical models, yet the e↵ectiveness of these models is often predicated on making simplifying approximations that allow for direct comparisons with available experimental data. The Michaelis-Menten approximation is widely used in both deterministic and discrete stochastic models of intracellular reaction networks, due to the ubiq...

The canard explosion phenomenon in a predator-prey model with Michaelis-Menten functional response is analyzed this paper by employing the geometric singular perturbation theory. First, some turning points, such as, fold point, transcritical pitchfork are identified; then Hopf bifurcation, relaxation oscillation, together transition from bifurcation to oscillation discussed.

Microbial bioanodes were formed in pulp and paper effluent on graphite plate electrodes under constant polarization at -0.3 V/SCE, without any addition of nutriment or substrate. The bioanodes were characterized in 3-electrode set-ups, in continuous mode, with hydraulic retention times from 6 to 48 h and inlet COD from 500 to 5200 mg/L. Current densities around 4A/m(2) were obtained and voltamm...

The immobilization of catalase was carried out from enzyme solutions in acid (pH < 3.5) and alkaline (pH > 1 1 ) medium on two kinds of soot differing by the average size of the particles. Under these conditions the peroxidase-like activity of immobilized catalase in the oxidation of phenol has been studied. The effect of the initial concentration of the substrate on the rate of the process cat...

Sucralose, one of the popular non-caloric artificial sweeteners, has been known to influence enzymatic conversion sucrose glucose and fructose by invertase. In continuing use real-time NMR experiments reaction progress curve analysis measure enzyme kinetics, here we investigate role sucralose as an inhibitor. based kinetic were performed a function substrate concentration for range concentratio...

We have made experimental studies into the enzymatic hydrolysis of cellobiose within the temperature range of 40°C to 70°C at pH 4.9, by using b-1,4-glucosidase from Aspergillus niger. At 70°C there was significant enzyme deactivation, which could be fitted to a potential deactivation model with values of n equal to 1.09 and kd to 0.1564 (g/l) 20.09 min, whereas the rate of hydrolysis could be ...

The electrogenicity and some molecular properties of the sarcoplasmic reticulum Ca2+ pump protein were studied by measuring steady-state Ca2+ pump currents. Ca2(+)-ATPase protein was solubilized from rabbit skeletal muscle sarcoplasmic reticulum membrane preparations and purified by liquid chromatography. The purified Ca(+)-ATPase molecules were reconstituted into proteoliposomes and then incor...

The oxidation of n-pentanol by tetramethylammonium fluorochromate in acidic solution wasstudied using spectrophotometric technique. The reaction was arranged to be under pseudo firstorderconditions respect to the oxidant. A Michaelis-Menten type kinetic was observed respect tothe substrate. The reaction is catalyzed by hydrogen ions. Dependences of the reaction rates ontemperature and different...

Studies were performed to investigate the UDP-glucuronosyltransferase enzyme(s) responsible for the human liver microsomal N2-glucuronidation of the anticonvulsant drug lamotrigine (LTG) and the mechanistic basis for the LTG-valproic acid (VPA) interaction in vivo. LTG N2-glucuronidation by microsomes from five livers exhibited atypical kinetics, best described by a model comprising the express...