The close functional interplay between the prosthetic group and the polypeptide chain in redox enzymes is the theme of this paper, and re— presents a natural extension of the well established regulation exerted by the protein mojety on the ligand binding properties of hemoglobins and other oxygen carriers. Two relevant examples are discussed: in the first one (azurin) the mole— cular mechanism ...

An inspection of the active site region of metallo-enzymes leads to two conclusions. (i) Individual attacking groups usually have uncommon physical properties. (ii) The active site region is cooperatively linked with a large part of the enzyme. Such an interaction is common to heterogeneous catalysts but is absent in homogeneous catalysts. It is concluded that the energetics of catalysis requir...

Here we describe a multiplexed electrochemical characterization of DNA-bound proteins containing [4Fe-4S] clusters. DNA-modified electrodes have become an essential tool for the characterization of the redox chemistry of DNA repair proteins containing redox cofactors, and multiplexing offers a means to probe different complex samples and substrates in parallel to elucidate this chemistry. Multi...