the taguchi design of experiments was used to test the relative importance of medium components and environmental factors on poly(β-hydroxybutyrate)(phb) production by ralstonia eutropha. the optimum condition was obtained as: fructose concentration, 15 g/l; c/n ratio, 7.4; agitation speed 200 rpm; culture time, 40 h; temperature, 25 ° c; seed age, 15 h. at optimum condition the yield of phb pr...

HeLa cells grow in a nutritionally complete synthetic medium (Ham's F12) supplemented with insulin, transferrin, hydrocortisone (aldosterone), fibroblast growth factor, and epidermal growth factor. This hormone-supplemented medium supports clonal growth, long-term cultivation, and a growth rate equal to that of serum-supplemented medium. The omission of any one of the five components results in...

A medium was developed for the differential enumeration of homofermentative and heterofermentative lactic acid bacteria. Essential components of the medium included fructose (14 mM), KH(2)PO(4) (18 mM), bromcresol green (as a pH indicator), and other nutrients to support growth. In agar medium, homofermentative colonies were blue to green, while heterofermentative colonies remained white. A tot...

The results of disk diffusion and plate dilution susceptibility testing of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis on media gelled with agar-agar or with a synthetic hydrogel were compared. Synthetic hydrogel can be combined with a totally defined synthetic amino acid medium to yield a reproducible, totally defined, synthetic solid medium without t...

A chemically defined medium was prepared which adequately supported growth of a vaccine strain of Pasteurella tularensis. This medium differed from those previously described in: (i) concentration of components, (ii) a requirement for calcium pantothenate to obtain increased growth, and (iii) a low initial pH. Varying the concentration of individual components up to 10 times the standard amount...

In nucleus-nucleus collisions, high-p(T) partons interact with a dense medium, which possesses strong collective flow components. Here, we demonstrate that the resulting medium-induced gluon radiation does not depend solely on the energy density of the medium, but also on the collective flow. Both components can be disentangled by the measurement of particle production associated with high-p(T)...

Plackett-Burman design was used to efficiently select important medium components affecting the lipase production by Aspergillus niger using shea butter cake as the main substrate. Out of the eleven medium components screened, six comprising of sucrose, (NH4)2SO4, Na2HPO4, MgSO4, Tween-80, and olive oil were found to contribute positively to the overall lipase production with a maximum producti...

to investigate the antifungal effects of essential oils of khuzestani savory (satureja khuzistanica jamzad), rechingeri savory (s. rechingeri jamzad), carvacrol and benomyl on botrytis cinerea, this study was carried out in a pda medium, and on grapes, kiwi fruits and strawberry. the experiment was laid out factorially based on a completely randomized design with three replications. the results...

Exopolysaccharides (EPSs) produced by Bifidobacteria have received considerable attention due to their ability to modify the rheological and physicochemical properties of dairy products. However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM). This study...