The directed search for extremophilic producers in order to obtain hydrolytic enzymes with increased thermal stability has an unconditional practical potential use the food and feed industry improve quality of final product. aim work was study ability collection strains thermophilic fungi show α-L-rhamnosidase, α-galactosidase, cellulase, β-mannanase, keratinase caseinolytic activity. Methods. ...

Endophytic fungi were isolated from the stems, petioles, midribs, and leaves of seedlings of six native tree species collected from Doi Suthep-Pui National Park, Thailand. Endophytes were isolated from all tissue samples investigated, and taxa included five ascomycetes, eight anamorphic taxa, and numerous sterile mycelia. Twenty-six strains were tested for their ability to produce cellulase, ma...

This study characterizes the glucomannan utilization operon (gmuBACDREFG, formerly ydhMNOPQRST) of Bacillus subtilis. Transcription of the operon is induced by konjac glucomannan and requires the last mannanase gene (gmuG). Cellobiose and mannobiose, possible degradation products of glucomannan by GmuG, are strong inducers of transcription. It is shown that an internal regulator gene (gmuR) enc...

The construction of minimum enzymes cocktails could facilitate the bioconversion of specified feedstocks into monosaccharides or XOS. In recent reported researches, the hemicelluloses and recombinant hemicellulases, including endo-ß-1,4-xylanases, ß-xylosidases, α-L-arabinofuranosidases, acetyl xylan esterase, feruloyl esterase and mannanase as well as their interaction mechanisms were investig...

High amounts of endo-β-mannanase (EC 3.2.1.78) activity were extracted from tomato (Lycopersicon esculentum Mill.) fruits when a high-salt-containing buffer was used. Two pI forms of the fruit enzyme were identified, one being much more basic than the many seed isoforms. The number of isoforms increased if a protease inhibitor was not used during extraction. The enzyme was found in the ripe fru...