pathogens such as Colletotrichum lagenarium and Magnaporthe grisea, melanization of appressoria is essential for penetration into the host plant.1) Fungal conidia germinate and the tips of germ tubes differentiate into appressoria, which synthesize melanin. The melanin mediates the buildup of pressure in the appressorium and this high pressure provides the essential driving force for mechanical...

Abstrak: Penghasilan enzim lignin peroksidase (LiP) dan mangan peroksidase (MnP) oleh Humicola grisea di dalam fermenter angkut udara diselidiki. Penghasilan optimum parameter yang digunakan pada fermenter ini ialah kadar pengudaraan 1.0 vvm, 1.0% (b/i) glukosa, 10.5 mM (NH4)2HPO4 dan kepekatan inokulum 1.0% (i/i; 6 × 10 spora/ml). Peningkatan sebanyak 52% dalam penghasilan LiP dan 49% dalam Mn...

Three new species of Paropesia Mesnil (Phasiinae, Parerigonini) are described: P. discalis sp. nov. from China and Myanmar, P. grisea sp. nov. from Japan and P. tessellata sp. nov. from Myanmar and China. The type species of Paropesia, P. nigra Mesnil, is redescribed. The male and female terminalia of the new species are described and illustrated and characteristics of the genus Paropesia are b...

More than thirty isolates of the thermophilic Torula-Humicola complex, mainly obtained from mushroom compost. were examined and compared with the available type cultures of Torula thermophila, Humicola grisea var. thermoidea and H. insolens. Scytalidium thermophilum is considered to be the correct name for all these isolates and type cultures, but the species is very variable in macroscopical a...

Magnaporthe grisea, the causal agent of rice blast disease, differentiates a specialized infection structure called an appressorium that is crucial for host plant penetration. Previously, it was found that cAMP regulates appressorium formation. To further understand the cellular mechanisms involved in appressorium formation, we have cloned a gene (MACl) encoding adenylate cyclase, a membrane-bo...

We have constructed a BAC library of the rice blast fungus Magnaporthe grisea consisting of 5760 clones. The insert size ranged from 35 to 175 kbp, with an average of 120 kbp. The library is about 18 genomes equivalent, therefore covering more than 99.999% of the genome. This library is the first to be constructed using a rice pathogenic wild type isolate. Improved high molecular weight DNA siz...

Outbreaks of rice blast have been a threat to the global production of rice. Members of the Magnaporthe grisea species complex cause blast disease on a wide range of gramineous hosts, including cultivated rice and other grass species. Recently, based on phylogenetic analyses and mating tests, isolates from crabgrass were separated from the species complex and named M. grisea. Then other isolate...

Black-grain mycetomas are subcutaneous devastating chronic infections due to several dematiaceous fungi. They are diagnosed mostly in tropical countries. Identification of these fungi with standard mycological procedures is difficult because of their poor or delayed sporulation. The aim of this study was thus to assess the accuracy of molecular identification of these fungi. A total of 54 strai...

Breeding crops with the quality of broad-spectrum disease resistance using genetic resources is one of the principal goals of crop improvement. However, the molecular mechanism of broad-spectrum resistance remains largely unknown. Here, we show that GH3-2, encoding an indole-3-acetic acid (IAA)-amido synthetase, mediates a broad-spectrum resistance to bacterial Xanthomonas oryzae pv oryzae and ...