نتایج جستجو برای: isolation mitochondria

تعداد نتایج: 176965  

Journal: :The Journal of Cell Biology 1965
M. Locke J. V. Collins

In the larva of the butterfly Calpodes ethlius, the fat body begins to store protein in the form of granules at about 30 to 35 hours before pupation, at a time when the endocuticle is being resorbed. At least two sorts of granule can be distinguished. The first granules to arise are those within vesicles of the Golgi complex. These may increase in size by incorporating material from microvesicl...

Journal: :Bio-protocol 2016
Thibaud T Renault Mark P A Luna-Vargas Jerry E Chipuk

The mitochondrial pathway of apoptosis involves a complex interplay between dozens of proteins and lipids, and is also dependent on the shape and size of mitochondria. The use of cellular models in past studies has not been ideal for investigating how the complex multi-factor interplay regulates the molecular mechanisms of mitochondrial outer membrane permeabilization (MOMP). Isolated systems h...

Journal: :The Journal of Biophysical and Biochemical Cytology 1955
Robert F. Witter Michael L. Watson Mary A. Cottone

Changes in the morphology of rat liver mitochondria brought about by different methods of isolation and the concomitant changes in ATP-ase activity were studied. The morphology was investigated with the electron microscope. It was found that the ATP-ase activity of the isolated mitochondria cannot be readily correlated with the morphology of the mitochondria. The ATP-ase found in these preparat...

Journal: :Plant physiology 1992
I Couée S Defontaine J P Carde A Pradet

Shoots of germinating rice (Oryza sativa L.) seedlings are able to grow under anoxia and to withstand long periods of anoxic treatment. Mitochondria were purified from aerobically germinated and anaerobically treated rice shoots by differential and isopycnic centrifugation and were found to consist of two subpopulations. The mitochondrial subpopulation of higher density was used for further cha...

Journal: :medical journal of islamic republic of iran 0
a. majid cheraghali from the department of pharmacology and toxicology, faculty of medicine, baghyatollah university of medical sciences, tehran, i.r. iran alan r.p paterson the department of pharmacology, university of alberta, edmonton, alberta, canada. wendy p. gati the department of pharmacology, university of alberta, edmonton, alberta, canada.

in this study the uptake and metabolism of adenosine by mitochondria has been investigated. incubation of cem cells mitochondria preparation with [3h] -adenosine showed substantial uptake and metabolism of adenosine. adenosine was both anabolized to amp, adp and atp, and also catabolized to inosine. the highest concentration of metabolites in extracted mitochondria was due to amp. the mitochond...

A. MAJID CHERAGHALI, ALAN R.P PATERSON, WENDY P. GATI,

In this study the uptake and metabolism of adenosine by mitochondria has been investigated. Incubation of CEM cells mitochondria preparation with [3H] -adenosine showed substantial uptake and metabolism of adenosine. Adenosine was both anabolized to AMP, ADP and ATP, and also catabolized to inosine. The highest concentration of metabolites in extracted mitochondria was due to AMP. The mito...

2017
Osamu Yamaguchi Tomokazu Murakawa Kazuhiko Nishida Kinya Otsu

Mitochondria are essential organelles that supply ATP through oxidative phosphorylation to maintain cellular homeostasis. Extrinsic or intrinsic agents can impair mitochondria, and these impaired mitochondria can generate reactive oxygen species (ROS) as byproducts, inducing cellular damage and cell death. The quality control of mitochondria is essential for the maintenance of normal cellular f...

Journal: :the iranian journal of pharmaceutical research 0
jalal pourahmad department of pharmacology and toxicology, school of pharmacy, shahid beheshti university of medical sciences tehran, iran. mir jamal hosseini department of pharmacology and toxicology, school of pharmacy zanjan university of medical sciences zanjan, iran.

mitochondria are unique organelles with major role in cellular function, not only as a major site of atp production, but also by regulating energy expenditure, apoptosis signaling, and production of reactive oxygen species. they play a central role in determining the point-of-no-return for the apoptotic process. there is accumulating evidence supporting a direct link between mitochondria, oxida...

Abdolmohammad Kajbafzadeh Anna Saffarpour Davood Sharifi Hamideh Salari Sedigh, Mahdi Ashouri Parviz Torkzaban Seyed Mahdi Nassiri Shahram Jamshidi,

Objective- Investigating of the isolation, culture, differentiation potential and electronic microscopic characterization of canine periodontal ligament stem cells (PDLSCs). Design- Experimental in vitro study Animals- Four intact, male, mongrel dogs, 8-10 months-old were selected to collect PDLSCs from their teeth. Procedures- The dogs were anesthetized and the first maxillary and mandibula...

Journal: :The Journal of Cell Biology 1972
H. Bruce Bosmann Marjorie W. Myers Delena Dehond Richard Ball Kenneth R. Case

N-acetylneuraminic acid at the surfaces of rat cerebral cortex and liver mitochondria and derived mitoplasts (inner membrane plus matrix particles) was studied biochemically and electrokinetically. Rat cerebral cortex mitochondria in 0.0145 M NaCl, 4.5% sorbitol, pH 7.2 +/- 0.1, 0.6 mM NaHCO(3), had an electrophoretic mobility of - 2.88 +/- 0.01 micro/sec per v per cm. In the same solution the ...

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