This article presents methods for the isolation and culture of epithelial cells from the bovine oviduct for use in both research and the teaching laboratory and provides examples of ways that an oviductal cell culture can be incorporated into an undergraduate research program. Cow reproductive tracts are readily available from area butchers, and the procedure for isolation of the epithelium is ...

No studies have quantified the impact of pre-culture antibiotic use on the recovery of individual blood-borne pathogens or on population-level incidence estimates for Streptococcus pneumoniae. We conducted bloodstream infection surveillance in Thailand during November 2005-June 2008. Pre-culture antibiotic use was assessed by reported use and by serum antimicrobial activity. Of 35,639 patient b...

Cell cultures are developed from tissue samples and then disaggregated by mechanical, chemical, and enzymatic methods to extract cells suitable for isolation of viruses. With the recent advances in technology, cell culture is considered a gold standard for virus isolation. This paper reviews the evolution of cell culture methods and demonstrates why cell culture is a preferred method for identi...

BACKGROUND New advances enable long-term organotypic culture of colonic epithelial stem cells that develop into structures known as colonoids. Colonoids represent a primary tissue source acting as a potential starting material for development of an in vitro model of the colon. Key features of colonic crypt isolation and subsequent colonoid culture have not been systematically optimized compromi...

To reduce culture artifacts by conventional repeated passaging and long-term culture in vitro, the isolation of synovial fibroblasts (SFB) was attempted from rheumatoid arthritis (RA) synovial membranes by trypsin/collagenase digest, short-term in vitro adherence (7 days), and negative isolation using magnetobead-coupled anti-CD14 monoclonal antibodies. This method yielded highly enriched SFB (...

background: in algeria, date palm is currently confronted to the bayoud disease. biotechnological tools such as protoplasts fusion can appear as an alternative to ensure rapid multiplication and improvement of this species.objectives: callogenesis induction in protoplasts isolated from embryogenic callus of three date palm cultivars.materials and methods: some factors influencing the isolation ...

nowadays, production of recombinant proteins in eukaryotes is gaining good deal of attention. transgenic chicken as a eukaryotic system has a high potential for producing recombinant proteins. post-translational changes, especially glycosylation, are characteristic of the eukaryotic proteins. in practice we need to choose a proper expressing host when considering over-expression of a recombinan...

the virulence of pasteurella multocida (p. multocida) serotype a1 for chickens and mice was determined. groups of chicken and mice were exposed intramuscularly and intraperitoneally to various concentration of p. multocida broth culture, respectively. this strain was highly virulent for chickens so that those exposed to only 7 c.f.u. of the organism died in less than 24 hours. groups of mice ex...

High levels of regulated oncogen-alpha (GRO-a) expression have been observed in the liver. GRO-a stimulates proliferation of epithelial cells and induction of rolling and extravascular migration of neutrophils and mononuclear cells. Given the above observations, this chemokine was chosen to be analyzed in freshly isolated and cultured hepatocytes. In this study, hepatocytes (2×106 cell/ml) were...