Two point mutations were identified within the gene encoding bovine CD18 in a Holstein calf afflicted with leukocyte adhesion deficiency (LAD). One mutation causes an aspartic acid to glycine substitution at amino acid 128 (D128G) in the highly conserved extracellular region of this adhesion glycoprotein, a region where several mutations have been found to cause human LAD. The other mutation is...

The European Community's (EC) Key, which is also called Bendixen's Key, is a well-established bovine leukemia virus (BLV) diagnostic method that classifies cattle according to the absolute lymphocyte count and age. The EC Key was originally designed for dairy cattle and is not necessarily suitable for Japanese Black (JB) beef cattle. This study revealed the lymphocyte counts in the BLV-free and...

The posttranscriptional gene regulation mediated by microRNA plays an important role in the development and function of male and female reproductive organs and germ cells in mammals, including cattle. In the present study, we identified novel and differentially expressed miRNAs in the testis and ovary in Holstein cattle by combining the Solexa sequencing with bioinformatics. In total 100 and 10...

The aim of this study was to refine the position of previously detected quantitative trait loci (QTL) on bovine chromosome 14 affecting milk production traits, using both linkage analysis (LA) and combined disequilibrium and linkage analysis (LDLA) methods, in Iranian Holstein dairy cattle. Analysis data was LRT (likelihood ratio test) and computed using the DMU (estimate the (co)variance compo...

Economic values of milk volume (VOL), fat yield (FYLD), protein yield (PYLD), live weight (LWT), longevity (LON), calving interval (CIV) and somatic cell score (SCS) were derived for Holstein and Jersey cattle, based on milk payment systems of four major milk buyers in South Africa. The economic value of somatic cell score was calculated for only two of the payment systems. A bio-economic model...

The International Society of Animal Genetics (ISAG) has chosen nine microsatellites (international marker set) as a standard that should be included in all cattle parentage studies. They are BM1824, BM2113, INRA023, SPS115, TGLA122, TGLA126, TGLA227, ETH10, and ETH225. We decided to ascertain whether this microsatellite set could be used to determine ancestral proportions in individual animals ...

Recent studies of mammalian genomes have uncovered the vast extent of copy number variations (CNVs) that contribute to phenotypic diversity. Compared to SNP, a CNV can cover a wider chromosome region, which may potentially incur substantial sequence changes and induce more significant effects on phenotypes. CNV has been becoming an alternative promising genetic marker in the field of genetic an...

CSN1S1is one of the major genes encoding milk proteins of mammals. In this study we determined allele frequencies of CSN1S1-5` flanking region as well as exon 17 variants and their effects on milk traits in three indigenous cattle breeds Mazandarani, Golpaygani (Bos indicus) and Sarabi (Bos taurus) and Holstein cattle in Iran. CSN1S1*B variant was nearly fixed in Holstein but ranged from 0.40 t...

mastitis is one of the most serious and costly diseases affecting dairy cattle production. in the present study, effects of a lactoferrin gene polymorphism (intron 6) on milk somatic cell count (scc) and subclinical mastitis was investigated in 121 holstein dairy cattle. two alleles of a and b and two genotypes of aa and ab were found in an ecori recognized single nucleotide polymorphism in int...