results analyzing of collected data resulted in the wrapping up the hepatitis virus detection methods in separate sections including 1) immunological methods such as enzyme immunoassay (eia), radio-immunoassay (ria) immuno-chromatographic assay (ica), and immuno-chemiluminescence 2) molecular approaches including non-amplification and amplification based methods, and finally 3) advanced biosens...

background: improving the performance of hiv diagnosis assays is one of the most important ways to reduce hiv transmission. because of the high mutation rate of hiv, it is critical to use the conserved proteins to develop diagnostic immunoassay methods. because integrase is one of the most conserved proteins of hiv, it may be a good target for this purpose. in this paper cloning, purification a...

BACKGROUND Despite the increase in sensitivity and specificity of immunoassay technique over years, analytical interference remains to be major area of concern. The interfering substances are endogenous substances that are natural, polyreactive antibodies as heterophilic or auto antibodies, or human anti-animal antibodies together with other unsuspected binding proteins that are unique to the i...

Here we report the development of a programmable and fully automatic gold array-embedded gradient microfluidic chip that integrates a gradient microfluidic device with gold-patterned microarray wells. This device provides a convenient and reproducible surface-enhanced Raman scattering (SERS)-based immunoassay platform for cancer biomarkers. We used hollow gold nanospheres (HGNs) as SERS agents ...

background and aims: some nanoparticles can be used in immunoassays to increase sensitivity. this study aimed to evaluate a novel nano-immunoassay based on bovine serum albumin nanoparticles (bsa nps). materials and methods: at first, the nanostructure was synthesized, and then applied as a tag in the nano-immunoassay. then the concentration of β-subunit of human chorionic gonadotropin (βhcg) i...

An electro-immunoassay technique was used to determine simultaneously immunoglobulin G (IgG) and albumin concentrations in serum and extracts of gingival tissue comprising the pocket wall. Assays of samples obtained from seven patients with juvenile periodontitis (mean age, 18 years) indicated that local synthesis accounted for 72% of the IgG found in the gingiva.

Plasma 13,14-dihydro-15-keto prostaglandin F2α (PGFM) and progesterone levels were determined in actively ovulating, 1to 2-yr-old female geese (Anser anser domesticus) at hourly intervals during the oviposition cycle, using the enzyme immunoassay (EIA) technique. The plasma PGFM concentration showed a peak at the time of oviposition and decreased to a basal level (

background: rotavirus is a major cause of morbidity and mortality among children with gastroenteritis. since the discovery of rotaviruses, several techniques have been used for their laboratory diagnosis; those included electron microscopy (em) and enzyme immunoassay. these methods, however, are expensive and not readily available everywhere. we have developed a technique which can be used for ...