Toxoplasmosis has been associated with economic and public health concerns due to abortion in humans animals. Detection for the presence of anti-Toxoplasma gondii antibodies sera 174 healthy sheep (IgG) 89 aborted women (IgG, IgM, IgG avidity) using enzyme-linked immunosorbent assay T. 59 milk samples identified by PCR. The primary risk factors seropositive were also analyzed. Relatively higher...

BACKGROUND Post-prandial worsening of symptoms as well as adverse reactions to one or more foods are common in the patients with functional gastrointestinal diseases, such as irritable bowel syndrome (IBS) and functional dyspepsia (FD). However, the role played by true food allergy in the pathogenesis of these diseases is still controversial and there are no well-established tests to identify f...

Study objective — assessment of the humoral and cell-mediated immunity features in COVID-19 convalescents three months after their discharge from hospital. Material Methods The study involved 78 who, depending on profile specific IgM IgG antibodies to SARS-CoV-2, were divided into groups. control group consisted 50 volunteers. Detection blood serum was performed by ELISA. Determination CRP conc...

A solid-phase radioimmunoassay developed in our laboratory for detection of human viral immunoglobulin M (IgM) and IgG antibodies was applied to demonstrate human class-specific antibody response against capsid, envelope, and excreted antigens of herpes simplex virus type 1. In primary infections, a clear IgM and IgG antibody response was found predominantly against the envelope components, whe...

Contemporary antiretroviral therapy (ART) is effective and tolerable for long periods of time but cannot eradicate human immunodeficiency virus type 1 (HIV-1) infection by either elimination of viral reservoirs or enhancement of HIV-1-specific immune responses. Boosting "protective" HIV-1-specific immune responses by active or passive immunization will therefore be necessary to control or eradi...

To use food-specific IgG antibody detection to explore its application in the allergy dermatoses. 181 patients were included from January 2014 to September 2014. Fourteen food-specific IgG antibodies were detected by ELISA. The positive rates of IgG antibody of the patient group and the healthy group were significantly different. The positive rates of IgG antibody of egg, milk, shrimp and crab ...

antibodies are a diverse class of glycoproteins that specially bind with antigen and elicit a number of secondary responses in vivo. most of the applications of antibodies in basic research diagnosis and immunotherapy rely on labeling of the antibody with radiopharmaceuticals. radiolabeled antibodies are a new class of imaging agents for the detection of sites of disease. true biodistribution o...

A "microgram assay" is described in which solid-phase enzyme-linked immunosorbent assay is used for the determination of specific rubella immunoglobulin G (IgG) antibody levels in micrograms per milliliter of serum. The quantitation was based on a standard curve obtained by using a reference serum, for which the specific IgG content was assayed by immunochemical purification. IgG was first puri...

BACKGROUND Hirudin is a small protein with strong thrombin inhibition that may be antigenic. The generation and disappearance of anti-hirudin antibodies were investigated in patients with heparin-induced thrombocytopenia who were treated with recombinant hirudin (r-hirudin) for >/=5 days. METHODS AND RESULTS The IgA, IgE, IgG, and IgM isotypes of anti-hirudin antibodies were determined by ELI...