The increasing demand for enantiopure drugs has led to the development of a variety of stereoselective separation technologies. Among them, high performance liquid chromatography (HPLC) is well recognized as a powerful, fast and efficient technique, which has been successfully employed for analysis and preparation of enantiomers of drugs. Nowadays, liquid chromatographic techniques are the focu...

Background and Objectives: A method was developed and validated for determining 15 polycyclic aromatic hydrocarbons (PAHs) in refined olive and refined pomace olive oils using high performance liquid chromatography coupled with a fluorescence detector. Materials and Methods: The sample preparation involved liquid–liquid extraction with organic solvent and low temperature clean-up. The low temp...

We assessed a relationship between the level of 8-oxodG in leukocyte DNA measured with the high performance liquid chromatography with electrochemical detection (HPLC/EC) technique and urinary excretion of the modified nucleoside/base analysed with a recently developed methodology involving HPLC prepurification followed by gas chromatography with isotope dilution mass spectrometric detection. N...

The carbobenzyloxy (cbz) protecting group is evaluated for it's potential to enhance the resolution of chiral amine enantiomers using high-performance liquid chromatography (HPLC) and supercritical fluid chromatography (SFC). A series of cbz derivatives of commercially available racemates was prepared and analyzed by enantioselective chromatography using a variety of mobile phases and polysacch...

apigenin is best known as an irreversible inhibitor of monoamine oxidase (mao), an intracellular enzyme associated with the outer membrane of mitochondria. the purpose of this study is to establish a reliable and quick method for the assignment of apigenin in cosmos bipinnatus, apium graveolens and petroselinum crispum by high performance liquid chromatography with ultraviolet detection (hplc-u...

This paper describes two rapid, sensitive and specific methods for the determination of fulvestrant in pharmaceutical preparations by high performance liquid chromatography (HPLC) and linear sweep voltammetry (LSV). HPLC method was used to study the degradation behaviour. Fulvestrant was subjected to degradation under the conditions of hydrolysis (acid and alkali), oxidation (30% H2O2). The lin...

results under the above chromatography condition, biuret compound (peqb) and the internal standard were detected at 4.5 and 13.5 minutes, respectively. limit of quantitation of the peqb was 0.1 µg/ml. accuracy of the method over the concentration range of 0.1-100 µg/ml was between 88-109%. inter- and intraday precisions were 4-19% and 6-8%, respectively. a good relationship in the form of a pow...

of isotopically labelled vitamin D metabolites by HPLC. Journal of Chromatography 303: 229}233. Kaspersen FM, van Acquoy J, van de Laar GLM, Wagenaars GN and Funke CW (1984) Deuterium isotope effects in mianserin. Recueil Travaux Chimique de Pay-Bas 103: 32}36. Klein PD (1966) Occurence and signiRcance of isotope fractionation during analytical separation of large molecules. Advances in Chromat...

Saraca asoca (Roxb.) De Wilde (Ashoka) is a highly valued endangered medicinal tree species from Western Ghats of India. Besides treating cardiac and circulatory problems, S. asoca provides immense relief in gynecological disorders. Higher price and demand, in contrast to the smaller population size of the plant, have motivated adulteration with other plants such as Polyalthia longifolia (Sonne...