Tryptic digestion of the diisopropyl phosphoryl-32P-Scarboxymethyl light chain derivative of human plasmin followed by gel filtration on Sephadex G-75 and G-25 and chromatography on Dowex SOW-X2 has resulted in the isolation of three radioactive peptides. These three peptides, having 31,33, and 34 residues, are all derived from the same segment of the plasmin molecule. Structural determinations...

Speract (Gly-Phe-Asp-Leu-Asn-Gly-Gly-Gly-ValGly), a Tyr analogue ("'yr-Asp-Leu-Asn-Gly-Gly-GlyVal-Gly), carboxyl-terminal fragment (Gly-Gly-GlyVal-Gly), and an NHz-terminaI fragment (Gly-Phe-AspLeu-Asn-Gly-Gly-Gly) were tested for their effects on sea urchin sperm respiration and net H+ efflux. The stimulation of net H' efflux by these peptides was highly correlated with their ability to sti...

A highly potent heart stimulant, anthopleurin A, from Anthopleura xanthogrammica was shown to exist as a single polypeptide chain consisting of 49 amino acid residues. The sequence of the peptide was shown to be: Gly-Val-Ser-Cys-Leu-Cys-Asp-Ser-Asp-Gly-Pro-Ser-Val-Arg-Gly-Asn-Thr-Leu-Ser-Gly-Thr-Leu-Trp-Leu-Tyr-Pro-Ser-Gly-Cys-Pro-Ser-Gly-Trp-His-Asn-Cys-Lys-Ala-His-Gly-Pro-Thr-Ile-Gly-Trp-Cys-...

Concentrations of glycine (Gly) in embryo culture media are often lower (~0.1 mM) than those in oviductal or uterine fluids (≥1.2 mM). The objective of this study was to determine direct and osmolarity-dependent effects of physiological concentrations of Gly on blastocyst formation and hatching, cell allocation to the trophectoderm (TE) and inner cell mass (ICM), and metabolic activity of bovin...