Crown-gall tumor tissue cultures release peroxidase into the medium in response to the concentration of specific ions in the medium. This release is not due to diffusion from cut surfaces or injured cells. Calcium, magnesium, and ammonium were, in that order, most effective in increasing peroxidase release. The enzyme was demonstrated cytochemically on the cell walls and in the cytoplasm. Cell ...

In recent years, many research studies have been conducted on the effects of magnetic field on plants. But certain mechanisms regarding magnetic influences have not been defined yet. In this study, effects of magnetic fields (MF) on the growth parameters and enzyme activities of Helianthus annuus L. were investigated. Sterilized seeds in sterile conditions were exposed to 0, 0.5, 1 and 1.5 mT o...

An indigenous wood rotting edible Lentinus kauffmanii isolated from Keeriparai forest of Western Ghats was studied for extracellular peroxidase production. Peroxidases are major extracellular lignolytic enzymes involved in lignocellulosic degradation. Physical factors such as pH, temperature and nutritional factors such as carbon, nitrogen sources and amino acids were standardized for the growt...

Horseradish peroxidase forms spectroscopically distinct, reversible complexes with hydroxamic acids (R-CONHOH), hydrazides (RCO-NHNH2), amides (RCONH2), and oc-hydroxyketones (RCO--CHsOH). Binding of these compounds to the enzyme depends on the polar and steric character of R and the hydrogen bonding capacity of-COX-Y (X-Y = NH-OH, NH-NH,, NH-H, CH,OH). Hydroxamate anions and hydrazide cations ...

Some Basidiomycota were chosen for studies of key ligninases synthesis (25°C, 30 days) in modified medium (shaken or not cultures) with added wheat straw. Liquid Czapek medium with straw yielded a higher amount of laccase than peroxidase, ground straw induced enzyme worse than chopped straw. With peroxidase the reverse dependencies were observed. Laccase of Lentinus edodes synthesized two enzym...

T HE ENZYME glutathione peroxidase appears to play a major role in the red cell in the detoxification of potential oxidizing agents. A genetically determined partial, or presumably heterozygous, deficiency of this enzyme has been associated with a transient hemolytic anemia and hyperbilirubinemia in newborns.l* Unless subjected to oxidative stress, red cells from older children and adults with ...

This article presents a reliable, effective and easy procedure for measuring glutathione peroxidase (Gpx) activity. Enzyme samples were incubated with phosphate buffer, which included the appropriate concentrations of (GSH) peroxide as substrates, to determine Gpx In CUPRAC method, reagent Cu(Nc)22+ was added stop enzymatic reaction after sufficient period incubation. The unreacted substrates a...

T HE ENZYME glutathione peroxidase appears to play a major role in the red cell in the detoxification of potential oxidizing agents. A genetically determined partial, or presumably heterozygous, deficiency of this enzyme has been associated with a transient hemolytic anemia and hyperbilirubinemia in newborns.l* Unless subjected to oxidative stress, red cells from older children and adults with ...