Classical models of gene expression were built using genetics and biochemistry. Although these approaches are powerful, they have very limited consideration the spatial temporal organization expression. dynamics RNA polymerase II (RNAPII) transcription machinery fundamental functional consequences for expression, its detailed studies been abrogated by limits classical light microscopy a long ti...

background: hox genes are well-known transcription factors that play essential roles in directing embryonic development. tgifly is a y-linked homeobox gene that was originally identified by virtue of its expression in adult testis. the functions of tgifly in normal and abnormal development are unknown. methods: to investigate the potential roles of tgifly gene in the infertile males, a semi-nes...

the pituitary-specific transcription factor (pit1) gene is responsible for pituitary development and hormone secreting gene expression in mammals. pit-1 is studied as a candidate genetic marker for growth, carcass and also for milk yield traits. in dairy farm animals, the principal goal of the selection is the improvement of milk yield and composition. the genes of milk proteins and hormones ar...

embryonic stem cells (escs) are pluripotent, self-renewing cells. these cells can be used in applications such as cell therapy, drug discovery, disease modelling, and the study of cellular differentiation. in this experimental study embryonic stem cells cultured in the laboratory and were amplified. total rna was extracted from cells and converted to cdna by reverse transcription-polymerase cha...

The sucrose non-fermenting 1-related protein kinase 2 (SnRK2) family members are plant unique serine/threonine kinases which play a key role in cellular signaling in response to abiotic stresses. The three SnRK2 members including SRK2D, SRK2I and SRK2E are known to phosphorylate major abscisic acid (ABA) responsive transcription factors, ABF2 and ABF4, involved in an ABA-dependent stress signal...

MXDs are transcription repressors that antagonize MYC-mediated gene activation. MYC, when associated with MIZ1, acts also as a repressor of subset genes, including p15 and p21. A role for in regulation MYC-repressed genes is not known. We report activate p21 U2OS cells. This activation required DNA binding by their interaction MIZ1. MXD mutants deficient MIZ1 interacted the MYC-binding partner ...

Objective: Ty2-917 is a low copy retrotransposon found in the Saccharomyces cerevisiae genome. It has structural similarities to metazoan retroviruses terms of genome organization and propagation mechanisms host cells. The objective this study analyze effects autophagy signaling on transcriptional regulation Ty2 yeast Materials Methods: Ty2-LacZ gene fusions YEp vectors have been used as report...