Microalgae are currently emerging as one of the most promising alternative sources for the next generation of food, feed, cosmetics and renewable energy in the form of biofuel. Microalgae constitute a diverse group of microorganisms with advantages like fast and efficient growth. In addition, they do not compete for arable land and offer very high lipid yield potential. Major challenges for the...

conclusion fm is more sensitive than zn for diagnosis of tb. however, since fm is more sensitive and rapid, using this method in clinical laboratories with large specimen numbers is recommended. results a total of 102 out of 920 study subjects had pulmonary tb, of them 68 (66.66%) patients were smear positive by either staining method while others were smear negative. the proportion of positive...

r-phycoerythrin (r-pe), a fluorescent protein from phycobiliprotein family, is isolated from red algae. conjugation of antibodies to r-pe facilitates multiple fluorescent staining methods. in the present study polyclonal antibodies and polyclonal f(ab')2 fragment antibodies were conjugated to r-pe by two different methods. the efficiency of the methods was evaluated using immunocytochemistry (i...

This is a preliminary report on a rapid fluorescent-antibody staining method utilizing the enzyme Bromelase that effectively eliminates nonspecific staining of staphylococci. By this simple method, group A streptococci were accurately identified from 2- to 4-h broth cultures.

A simple and sensitive post electrophoresis fluorescent staining technique for proteins on polyacrylamide gels using 5-dimethylamino-1-naphthalene sulfonyl chloride (dansyl chloride) has been developed. Dansyl chloride staining increases the sensitivity, 0.125 micrograms protein per band can be visualised by this technique. The staining method appears to be applicable to all types of proteins i...

Fluorescent cellular barcoding and mass-tag cellular barcoding are cytometric methods that enable high sample throughput, minimize inter-sample variation, and reduce reagent consumption. Previously employed barcoding protocols require that barcoding be performed after surface marker staining, complicating combining the technique with measurement of alcohol-sensitive surface epitopes. This repor...