Human renal carcinoma cell line GKA was derived from a patient with the paraneoplastic syndrome of erythrocytosis and secretes erythropoietin-like activity into its growth medium (Sytkowski, A. J., Richie, J. P., and Bicknell, K. A. Cancer Res., 43: 1415-1419, 1983). In order to derive homogeneous sublines with higher secretory rates, we cloned line GKA. Over 100 clones were generated, and 21 s...

to accomplish the worldwide demand for recombinant human erythropoietin (rhuepo) as a therapeutic, application of cost-efficient expression system of methylotrophic yeast pichia pastoris (p. pastoris) rather than mammalian cells is indispensable. herein, a report on high levels secreted-expression of pichia-derived rhuepo by batch fermentation in a ph stabilized format is presented. the full le...

purpose : to determine the maximum non-toxic dose of recombinant human erythropoietin (epo) in rabbit eyes methods : eight rabbits (sixteen eyes) were scheduled for evaluating side effects of four intravitreal doses of 1000, 2000, 4000 and 5000 iu of epo, two rabbits for each dose. for each dose (two rabbits), the drug was injected for the right eyes. balanced salt solution (bss) was injected i...

conclusions epo injection into the site of tibiofibular fractures may possibly accelerate healing. background erythropoietin (epo), in addition to its function as an erythropoiesis regulator has a regenerative activity on some nonhematopoietic tissues. animal studies have suggested a role for erythropoietin in bone healing. objectives the present study aimed to evaluate the effects of local epo...

background: vast variety of intermediate factors including cell cycle regulators, growth factors, transcription factors, and signaling pathways are involved in hematopoietic stem cell (hsc) commitment and differentiation into distinct lineages. vhl, ecad, and runx3 are among these. epigenetics is currently introduced as a potential mechanism to control the gene regulation. the aim of this study...

Via sites 1 and 2, erythropoietin binds asymmetrically to two identical receptor monomers, although it is unclear how asymmetry affects receptor activation and signaling. Here we report the design and validation of two mutant erythropoietin receptors that probe the role of individual members of the receptor dimer by selectively binding either site 1 or site 2 on erythropoietin. Ba/F3 cells expr...

Erythropoietin or colony-stimulating factor, or both, were added to rat or mouse marrow cell cultures, and the responses to each inducer were measured. Colony-stimulating factor caused the suppression of erythropoietin-stimulated hemoglobin synthesis, and erythropoietin caused the suppression of the granulocyte-macrophage colony formation that is dependent on colony-stimulating factor. The exte...

Cultures initiated with isolated goat-kidney glomeruli produce erythropoietin over periods of 7 months. Production seems dependent upon a minimum interval of about 30 days between changes of medium. Experiments distinguishing between erythropoietin, renal activator, and serum substrate support the conclusion that the factor released to the medium of long-term glomerular cultures is erythropoiet...

I n addition to its role as the main regulator of erythropoiesis, erythropoietin has a wide range of protective, antiapoptotic activities in vitro and in vivo, particularly in the brain and, as more recently shown, in the heart. Of note, erythropoietin is not exclusively produced by fetal liver and adult kidney but is expressed also in the brain, where it can be induced by hypoxia or ischaemia....

Gentamicin nephrotoxicity limit its usage against gram negative bacteria. Most researches showed that antioxidant agents improved gentamicin nephrotoxicity. According to these investigations oxidative stress play a central role in the mechanism of gentamicin induced nephrotoxicity. Recently Rafieian-Kopaei and colleagues showed that erythropoietin significantly ameliorated serum creatinine, blo...