The reverse-phase high-performance liquid chromatography elution data, expressed in glucose units, of 2-pyridylaminated oligosaccharides (PA-oligosaccharides) originated from N-glycosides of glycoproteins have been analyzed with multiple regression to obtain parameters of contribution ascribable to each monosaccharide unit. For the best results, the known PA-oligosaccharides were classified int...

The separation of compounds by chromatography is often followed by the need to separate the compounds from the stationary phase for quantification, rechromatography, or other analytical procedures. In thin-layer chromatography compounds are usually separated from the adsorbent by removal of the layer from the backing by scraping or suction, followed by elution of the compounds in columns or by ...

OBJECTIVES To assess the performance of the Gram-negative-specific antibiotic temocillin in polymethylmethacrylate bone cement pre-loaded with gentamicin, as a strategy for local antibiotic delivery. METHODS Temocillin was added at varying concentrations to commercial gentamicin-loaded bone cement. The elution of the antibiotic from cement samples over a 2 week period was quantified by LC-MS....

-Gradient elution in nonlinear chromatography is a very powerful tool for the separation of components having a wide range of retentivity. It is not only used for chemical analysis, but also used in preparativeand large-scale chromatography for the separation of many macromolecules, such as proteins. In this work, a general rate model has been presented for the study of gradient elution in nonl...

Background: Technetium-99m radioisotope is the backbone of any nuclear medicine institute. This radioisotope is acquired by the elution of Molybdenum-99/Technetium-99m generator which emits 141 keV gamma ray and having 6.04 hour half-life. Occasionally the contents of Molybdenum-99 may mix up with elute, which may affect image quality and patients may receive higher doses than recommended. This...

Determination of antibody avidity measurements can be difficult in human serum depending on the population evaluated. We evaluated three approaches for the determination of antibody avidity for immunoglobulin G (IgG). These approaches were (i) elution of bound antibody with increasing concentrations of a chaotropic agent using a single serum dilution, (ii) binding interference of multiple serum...