background: the ability to sensitively detect vibrio cholera with pcr-elisa method represents a considerable advancement over alternative more time-consuming methods for detection of this pathogen. the aim of this research is to evaluate the suitability of a pcr-enzyme-linked immunosorbent assay for sensitive and rapid detection of v. cholera o1. methods: the 398-bp sequence of a gene that code...

b ackground: toxoplasmosis is a serious disease in immunocompromised patients and pregnant women. differentiation of acute and chronic infection is a major challenge in serodiagnosis of the disease. since the aim of this study was to assess the diagnostic utility of recombinant sag1 (rec- sag1) for the detection of toxoplasma -specific igm antibodies in human sera, by an enzyme-linked immunosor...

Background: The ability to sensitively detect Vibrio cholera with PCR-ELISA method represents a considerable advancement over alternative more time-consuming methods for detection of this pathogen. The aim of this research is to evaluate the suitability of a PCR-enzyme-linked immunosorbent assay for sensitive and rapid detection of V. cholera O1. Methods: The 398-bp sequence of a gene that cod...

introduction: the development of an effective vaccine against hepatitis c virus (hcv) is still a target of intensive vaccine research. the hcv envelope proteins e1 and e2 which can induce broadly neutralizing antibodies are the major candidate for this purpose. different types of expression systems have been used to express these glycoproteins. in this study, an expression system using pichia p...

Lennefors, B-L. 2006. Molecular breeding for resistance to rhizomania in sugar beets. Doctor’s dissertation ISSN: 1652-6880, ISBN: 91-576-7255-5 Rhizomania is one of the most destructive sugar beet diseases. It is caused by Beet necrotic yellow vein virus (BNYVV) vectored by the soilborne protoctist Polymyxa betae Keskin. The studies in this thesis evaluated natural and transgenic resistances t...

isolation and differentiation of various species of mycobacteria are predominantly based on conventional, cultural and biochemical tests. among the non-tuberculous mycobacteria, these tests are unable to differentiate m. scrofulaceum from m. avium-intracellulare (mai) because of their identical biochemical characteristics. for this reason, one hundred mycobacterial strains suspected to be m. sc...

Many methods have been developed and applied to diagnose plant viruses. Some of these methods are biological (e.g. indexing on a susceptible plant genotype), others are serological (enzyme-linked immunosorbent assay [ELISA] and lateral flow test carried out with either polyclonal antisera or monoclonal antibodies) or molecular (nucleic acid hybridization, PCR or real time PCR). Most of diagnost...