نتایج جستجو برای: denaturing
تعداد نتایج: 4945 فیلتر نتایج به سال:
The protocols for in situ hybridization (ISH) techniques can vary considerably; however, they usually include denaturation and steps. Denaturing compounds are used to reduce temperature, which keeps the proper morphology of preparation. Formamide is most commonly reagent lower melting temperature. substitution toxic formamide a non-toxic ethylene carbonate at 20 % 50 concentration mixture helpe...
Tandem affinity strategies reach exceptional protein purification grades and have considerably improved the outcome of mass spectrometry-based proteomic experiments. However, current tandem affinity tags are incompatible with two-step purification under fully denaturing conditions. Such stringent purification conditions are desirable for mass spectrometric analyses of protein modifications as t...
The experiments described in this paper show (I) that the oxidation of SH groups by common oxidizing agents such as ferricyanide and Folin's uric acid reagent is inhibited by cyanide and promoted by copper sulfate, (2) that the SH groups of denatured egg albumin can be oxidized by an equivalent amount of ferricyanide even in the absence of denaturing agents, provided aggregation is avoided, (3)...
The experiments described in this paper show (I) that the oxidation of SH groups by common oxidizing agents such as ferricyanide and Folin's uric acid reagent is inhibited by cyanide and promoted by copper sulfate, (2) that the SH groups of denatured egg albumin can be oxidized by an equivalent amount of ferricyanide even in the absence of denaturing agents, provided aggregation is avoided, (3)...
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