Understanding patterns of species diversity relies on accurate taxonomy which can only be achieved by long-term natural history research and the use of complementary information to establish species boundaries among cryptic taxa. We used DNA barcoding to characterize the ant diversity of Iguazú National Park (INP), a protected area of the Upper Paraná Atlantic Forest ecoregion, located at the s...

BACKGROUND AND AIMS Fishes are the main animal protein source for human beings and play a vital role in aquatic ecosystems and food webs. Fish identification can be challenging, especially in the tropics (due to high diversity), and this is particularly true for larval forms or fragmentary remains. DNA barcoding, which uses the 5' region of the mitochondrial cytochrome c oxidase subunit I (COI)...

The bumblebee, Bombus ignitus (Hymenoptera, Apidae), plays a vital role in pollination Northeast Asia, including Korea, China, Japan, and Far East Russia. Understanding the genetic makeup of population can aid its conservation. This study explores DNA barcode region cytochrome C oxidase subunit I (COI) B. commercial populations from Korea Japan. results reveal low intraspecific diversity among ...

For sponges (phylum Porifera), there is no reliable molecular protocol available for species identification. To address this gap, we developed a multilocus-based Sponge Identification Protocol (SIP) validated by a sample of 37 sponge species belonging to 10 orders from South Australia. The universal barcode COI mtDNA, 28S rRNA gene (D3-D5), and the nuclear ITS1-5.8S-ITS2 region were evaluated f...

BACKGROUND DNA barcoding has recently been proposed as a promising tool for the rapid species identification in a wide range of animal taxa. Two broad methods (distance and monophyly-based methods) have been used. One method is based on degree of DNA sequence variation within and between species while another method requires the recovery of species as discrete clades (monophyly) on a phylogenet...

Soil nematodes play crucial roles in the soil food web and are a suitable indicator for assessing soil environments and ecosystems. Previous nematode community analyses based on nematode morphology classification have been shown to be useful for assessing various soil environments. Here we have conducted DNA barcode analysis for soil nematode community analyses in Japanese soils. We isolated ne...

Chrysomelidae on the islands of Vietnam has been poorly known. In this study, we investigated diversity Phu Quoc Island in Kien Giang province, Southern Vietnam. Specimens were collected from national park and buffer zone forest. First, all specimens ordered into a morph-species operational taxonomic unit (OTU). We 52 morphological OTUs 31 genera 5 subfamilies, 20 which identified as level spec...

Development of effective conservation and management strategies requires assessments ecosystem biodiversity status, especially in understudied hotspots global fish diversity. Coral reefs are important habitats for fishes, with known globally. We present the first data on molecular diversity fishes Mischief Reef, largest atoll Nansha Islands. Partial sequences (650 bp) mitochondrial COI gene (Cy...

Aim: The aim of this study was to assess the biodiversity and community structure of Swedish meiofaunal eukaryotes using metabarcoding. To validate the reliability of the metabarcoding approach, we compare the taxonomic resolution obtained using the mitochondrial cytochrome oxidase 1 (COI) ‘mini-barcode’ and nuclear 18S small ribosomal subunit (18S) V1-V2 region, with traditional morphology-bas...

Two new species of Sycorax Haliday in Curtis, 1839 are described from Ulu Temburong National Park in Brunei Darussalam: Sycorax konopiki sp. nov. and S. tomkineana sp. nov. Both species were found resting on two frog species: Ansonia leptopus (Günther, 1872) and A. longidigita Inger, 1960. Differential diagnoses for males are included and morphological characters illustrated. Possible host asso...