نتایج جستجو برای: clone library

تعداد نتایج: 156567  

Journal: :Journal of environmental sciences 2007
Chun-bo Hao Hong-xun Zhang Zhi-hui Bai Qing Hu Bao-guo Zhang

Waste ore samples (pH 3.0) were collected at an acid mine drainage (AMD) site in Anhui, China. The present acidophilic microbial community in the waste ore was studied with 16S rRNA gene clone library and denaturing gradient gel electrophoresis (DGGE). Eighteen different clones were identified and affiliated with Actinobacteria, low G + C Gram-positives, Thermomicrobia, Acidobacteria, Proteobac...

Journal: :Journal of cell science 1990
R Kuriyama P Savereide P Lefebvre S Dasgupta

Monoclonal antibody (SU5), prepared from isolated mitotic spindles of sea urchin eggs, stained centrospheres preferentially and recognized a 50K (K = 10(3) Mr) polypeptide on immunoblots. Three positive clones were isolated by screening a lambda gt11 cDNA expression library prepared from sea urchin egg mRNA with SU5. One clone containing a 1.8-kb (1 kb = 10(3) base-pairs) insert was selected fo...

Journal: :BioTechniques 2002
A Dobi W Debnam C Dalgard A Owusu D von Agoston

Here we report a functional screening technique to identify cDNAs encoding mammalian nucleic acid binding proteins. We have combined cDNA expression cloning with the agarose thin-layer gelshift assay technique to detect specific nucleic acid binding proteins from a mammalian expression library. We divided this cDNA expression library into multiple pools and transfected mammalian cells with the ...

Journal: :Plant physiology 1998
I Vijn A van Dijken M Lüscher A Bos E Smeets P Weisbeek A Wiemken S Smeekens

Sucrose (Suc):Suc 1-fructosyltransferase (1-SST) is the key enzyme in plant fructan biosynthesis, since it catalyzes de novo fructan synthesis from Suc. We have cloned 1-SST from onion (Allium cepa) by screening a cDNA library using acid invertase from tulip (Tulipa gesneriana) as a probe. Expression assays in tobacco (Nicotiana plumbaginifolia) protoplasts showed the formation of 1-kestose fro...

Journal: :Gene 1984
V N Ivanov D R Kershulite A A Bayev A A Akhundova G E Sulimova E S Judinkova S I Gorodetsky

A sensitive immunoassay was used to identify recombinant plasmids carrying cDNA fragments of bovine caseins in the cDNA library from bovine mammary gland mRNA. Colonies grown on nitrocellulose filters were lysed in situ and proteins from the lysates were blotted onto CNBr-activated cellulose filter paper. Antigens covalently bound to CNBr-activated paper or bound to nitrocellulose filters were ...

2011
Man Cheng Shirley Y. W. Chan Qi Zhao Elaine Y. M. Chan Shannon W. N. Au Susanna S. T. Lee Wing-Tai Cheung

Antibody repertoires for library construction are conventionally harvested from mRNAs of immune cells. To examine whether germline rearranged immunoglobulin (Ig) variable region genes could be used as source of antibody repertoire, an immunized phage-displayed scFv library was prepared using splenocytic genomic DNA as template. In addition, a novel frame-shifting PCR (fsPCR) step was introduced...

Journal: :Gene 1985
W Lindenmaier K E Dittmar H Hauser A Necker W Sebald

A method has been developed that allows the isolation of genomic clones from a cosmid library by homologous recombination in vivo. This method was used to isolate a human genomic interleukin 2 (IL2) gene. The genomic cosmid library was packaged in vivo into lambda phage particles. A recombination-proficient host strain carrying IL2 cDNA sequences in a non-homologous plasmid vector was infected ...

Journal: :Clinical and vaccine immunology : CVI 2006
Jinye Liu Hongxia Shao Yanlin Tao Bin Yang Lisheng Qian Xiaoli Yang Brian Cao Gengxi Hu Hiroshi Tachibana Xunjia Cheng

A combinatorial human immunoglobulin gene library was constructed from the peripheral lymphocytes of two patients who recovered from severe acute respiratory syndrome (SARS). The library was screened for the production of Fab antibody fragments to a recombinant spike protein of SARS-associated coronavirus (SARS-CoV). One Fab clone, AS3-3, reacted with the spike protein in an enzyme-linked immun...

Journal: :Journal of microbiology and biotechnology 2014
Bo Xu Fuya Yang Caiyun Xiong Junjun Li Xianghua Tang Junpei Zhou Zhenrong Xie Junmei Ding Yunjuan Yang Zunxi Huang

To isolate novel and useful microbial enzymes from uncultured gastrointestinal microorganisms, a fecal microbial metagenomic library of the pygmy loris was constructed. The library was screened for amylolytic activity, and 8 of 50,000 recombinant clones showed amylolytic activity. Subcloning and sequence analysis of a positive clone led to the identification a novel gene (amyPL) coding for α-am...

Journal: :Applied and environmental microbiology 2006
Carl B Abulencia Denise L Wyborski Joseph A Garcia Mircea Podar Wenqiong Chen Sherman H Chang Hwai W Chang David Watson Eoin L Brodie Terry C Hazen Martin Keller

Low-biomass samples from nitrate and heavy metal contaminated soils yield DNA amounts that have limited use for direct, native analysis and screening. Multiple displacement amplification (MDA) using phi29 DNA polymerase was used to amplify whole genomes from environmental, contaminated, subsurface sediments. By first amplifying the genomic DNA (gDNA), biodiversity analysis and gDNA library cons...

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