A monolithic silica column has recently been widely used for HPLC separation analysis. Compared with the particle−packed column (conventional column), the monolithic silica column eluted the analyte with a smaller retention factor (k), lower pressure and satisfactory separation [1]. However, the monolithic silica column has not been widely used for the HPLC or LC/MS analysis of steroids. These ...

A simple, precise and accurate reversed-phase liquid chromatographic method has been developed for the simultaneous estimation of Rosuvastatin and Fenofibrate in tablet formulations. The chromatographic separation was achieved on (Inertsil ODS, 250 x 4.6mm, 5μ column) analytical column. And mobile phase as mixture of Water (pH adjusted to 2.5 with ortho phosphoric acid) and acetonitrile in rati...

The preparation of quantities of poly(ADP-ribose) glycohydrolase sufficient for detailed structural and enzymatic characterizations has been difficult due to the very low tissue content of the enzyme and its lability in late stages of purification. To date, the only purification of this enzyme to apparent homogeneity has involved a procedure requiring 6 column chromatographic steps. Described h...

One of the ultimate goals of ion chromatography is to determine both anions and cations found in samples with a single chromatographic run. In the present article, recent progress in ion-exclusion/ion-exchange chromatography for the simultaneous determinations of inorganic anions and cations are reviewed. Firstly, the principle and the control for the simultaneous separation and detection of ...

We have developed a stable and simple normal-phase liquid-chromatographic method for simultaneously measuring amiodarone and its metabolite, N-desethylamiodarone, within 8 min. The chromatographic system consists of a 15 cm x 3.9 mm Waters "Resolve" silica column and a mobile phase of ammonium sulfate (17 mmol/L, pH 6.8) and methanol (8/92 by vol), pumped at 1.8 mL/min and monitored at 254 nm. ...

Electrophoretic and isoelectric focusing studies have demonstrated the presence of multiple sub-forms of the major creatine kinase isoenzymes, and preliminary work has suggested that measurement of the mm sub-forms provides early diagnostic information concerning acute myocardial infarction. We developed an isocratic method based on liquid chromatography to examine further the clinical potentia...

The axial dispersion coefficient of hexachlorobenzene in supercritical carbon dioxide is investigated in a fixed-bed packed with glass beads. The on-line chromatographic pulse-response experiment is used in order to study the dynamics of a packed column under supercritical conditions. The radial dispersion is assumed negligible because of the packed column geometry. To estimate the axial disper...

A simple and rapid reversed-phase high-performance liquid-chromatographic method for the separation and determination of process-related impurities of celecoxib (CXB) in bulk drugs and pharmaceuticals was developed. The separation of impurities viz., 4-methylacetophenone (I), 1-(4-methylphenyl)-4,4,4-trifluorobutane-1,3-dione (II), 4-hydrazinobenzene sulfonamide (III) and a regio-specific isome...

The supernatants of the homogenates from normal and cancerous human prostatic tissue run on polyacrylamide gel electrophoresis have 2 major electrophoretic bands when stained for prostatic acid phosphatase. The ratios of the electrophoretically distinguishable isoenzymes differ in normal and cancerous tissues. Similar distinctions between isoenzymes in normal and cancerous prostates are observe...

In a case of fatal paraquat poisoning, the distribution of paraquat was determined by a) the column chromatographic method developed by Tompsett, b) the ion-pairing method, c) the deproteinization method both developed by Jarvie et al, and d) the precipitation method developed by the authors. The precipitation method relies on the use of a precipitate of paraquat with Reinecke's salt to extract...