Background: T-2 toxin is a mycotoxin of type A trichothecenes produced by several fungal genera such as Fusarium species. Mycotoxins can affect both cell mediated and humoral immune compartments. Objective: The purpose of this study was to investi-gate the effect of T-2 toxin on cytokine production by mouse peritoneal macrophages and lymph node T cells. Methods: Mouse peritoneal macrophages and...

1. Abstract 2. Introduction 3. Material and methods 3.1. Cloning and sequencing of TYchi gene 3.2. Southern and northern blot hybridization 3.3. Expression and Purification of Recombinant TYchi 3.4. Chitinase enzymic assay of TYchi 3.5. Assay for RNA N-glycosidase activity 3.6. Assay for cell-free translation-inhibiting activity 3.7. Assay for cytotoxic activity on human tumor cells 3.8. Modeli...

Rotavirus recombinant vaccine is usually produced in Vero cells. Residual host DNA may reside the final product and considered a source of contamination. WHO protocols indicate that biological products should be free any type impurity such as nucleic acids, endotoxins, cell intermediate materials. Therefore, all therapeutics assessed for residual DNA. In present study, sensitive specific real-t...

abstract background: radiosensitizer drugs are used to enhance the efficiency of radiotherapy. some nanoparticles can be considered as radiosensitizers, because they enhance cytotoxicity due to oxidative stress and increase free radical yield, especially ros , within cells resulting to cell death. methods: in this study, synergistic effect of tio 2 nanoparticles was evaluated in presence of 60 ...

background: various mtt assay methods are proposed to obtain the cell sur- vival parameters. objective: determining the survival curve characteristics of two cancerous cells of interest based on a common and a novel mtt assay method after exposing them to ionizing radiation. method: a common and a novel mtt assay method were used and compared for obtaining the f10b16 melanoma and 4t1 breast ade...

background: natural medicines have been recently considered more reasonable for human use most notably due to their safety and tolerance. hesa-a is a marine-originated herbal medicine with a variety of healing effects. however, its exact biological mechanism is not clear. the pre-sent study aimed at the evaluation of the hesa-a antioxidant effect. methods: chinese hamster ovary (cho) and human ...