310 CMYK Figure 2: (a) Calcofluor white stain of the corneal scraping showing septate, branched fungi with chlamydospores. (x250). (b) Eight day old growth of the fungus on potato dextrose agar showing Grey, fluffy colonies with black granules on the surface. (c) Lactophenol cotton blue wet mount of the colony showing falcate conidia (shown with arrowheads), and conidiomata, upon which darkly p...

The viability of algae belonging to divisions Cyanophyta, Chlorophyta and Xantophyta has been assessed by chlorophyll autofluorescence and after staining with fluorescent dyes rodamin B, neutral rot, calcofluor white and fluorescein diacetate. For this purpose the fluorescence of algal cells from fresh and old cultures was scored. It was established that chlorophyll autofluorescence could be re...

Campylobacter jejuni helical shape is important for colonization and host interactions with straight mutants having altered biological properties. Passage on calcofluor white (CFW) resulted in C. jejuni 81-176 isolates with morphology changes: either a straight morphology from frameshift mutations and single nucleotide polymorphisms in peptidoglycan hydrolase genes pgp1 or pgp2 or a reduction i...

An 81-year-old female presented with a three year history of painless, erythematous nodules on her right proximal forearm slow spread to the cubital fossa and distal upper arm. A 78-year-old six month isolated plaque. The patient had background medical inflammatory arthritis managed oral prednisone infliximab. In both cases calcofluor-white stain revealed organisms resembling Prototheca. Growth...

Paper production and recycling result in large amounts of recycled paper sludge (RPS) that is currently being disposed very costly unsustainable practices, raising the importance developing green solutions for waste management. The use nanocellulose (NC) as next generation materials has gained much attention due to its economic potential. However, there are substantial challenges NC extraction,...

A prospective study of intestinal microsporidiosis in HIV-positive children was conducted at the Queen Sirikit National Institute of Child Health and Phramongkutklao Hospital, Bangkok, Thailand. Hospitalized HIV-positive children with and without diarrhea were enrolled in this study. Microsporidial spores identified by calcofluor fluorescent and gram-chromotrope stain were confirmed by electron...

We identified a nonpathogenic strain of Ustilago maydis by tagging mutagenesis. The affected gene, glucosidase1 (gas1), displays similarity to catalytic alpha-subunits of endoplasmic reticulum (ER) glucosidase II. We have shown that Gas1 localizes to the ER and complements the temperature-sensitive phenotype of a Saccharomyces cerevisiae mutant lacking ER glucosidase II. gas1 deletion mutants w...