Valuable products obtainedfrom the cultivation of anchorage-dependent mammalian cells require large-scale processes to obtain commercially useful quantities. It is generally accepted that suspension culture is the ideal mode of operation. Because anchorage-dependent cells need surfaces to be able to attach and spread, the incorporation of microcarriers to suspension culture is indispensable. Si...

Natural biopolymers are widely used in the field of drug and gene delivery. In this study, alginate nanoparticles were prepared using water-in-oil reverse microemulsion as a template followed by calcium crosslinking of guluronic acid units of alginate polymer. After collected by ultracentrifugation, alginate nanoparticles were analyzed by electron microscopy to obtain the size and morphology wh...

the purpose of the research was to formulate microspheres of acyclovir (acv) using mucoadhesive polymers, sodium alginate and chitosan. calcium chloride was used as the ionotropic gelling agent. sodium alginate was crosslinked by calcium chloride leading to a slower release of the drug. chitosan which is a cationic polymer interacted with sodium alginate, an anionic polymer, to form an interpol...

Wound dressings play important roles in the management of wounds, and calcium cross-linked alginate (Ca2+-Alg) is a commonly used hydrogel that is adapted for wound treatment. However, conventional methods for fabricating Ca2+-Alg hydrogels can be tedious and difficult to control because of the rapid Ca2+-induced gelation of alginate. In this study, An electrodeposition method was used to rapid...

Encapsulation techniques are fmding increasing interest in a variety of areas of biotechnology. Enzymes, living ceils, hormones, drugs, adsorbents and other bioactive materials can be encapsulated and used in bioreactors, artificial organs, controlled drug-release systems, bioseparation systems and etc (Chang, 1972; Kondo, 1979; Lira, 1984; Nigam, 1988; Nixon, 1976). Ideally, encapsulation of b...

Calcium-induced alginate gel beads (Alg-Ca) containing various polysaccharides, including an alginate hydrolysate, were prepared and the drug release profiles were investigated. Hydrocortisone (HC) was gradually released from Alg-Ca into the mimic gastric fluid, while in intestinal fluid, it was quickly released with the dissolution of Alg-Ca. However, with Alg-Ca containing 5% chitin (CT), dis...

The comparative study of food fibers preparations (“Vitacel” – wheat fiber, beet fibers, calcium alginate, TIPRO 601 – native collagen protein, pork rin d) in a chronic biological experiment has shown that none of them had cumulative toxic effect, and possessed a low level of food sensibilization. Calcium alginate, native collagen protein and pork rind help to increase calcium content in bones ...

Gelation speed is directly proportional to the concentration of calcium. Although the kinetics of gelation are altered by the source of calcium, the final alginate gel strength nor the resistance to calcium diffusion are altered. Calcium chloride reaches a gel strength plateau fastest ( 100 s), followed by calcium lactate ( 500 s) and calcium gluconoate ( 2000 s). Calcium chloride is the best o...