The aim of this study was to investigate the effects of pinocembrin on brain ischemia/reperfusion (I/R) injury and the potential involvement of autophagy activity changes in the penumbra area in the mechanisms of pinocembrin activity. Focal cerebral I/R model was induced by middle cerebral artery occlusion (MCAO) for 2 h followed by 24 h reperfusion. Pinocembrin was administered intravenously a...

s were prepared by the authors and printed as submitted. T H U R S D A Y, JU N E 2, 2016 was carried out in the third group (DM-EX group) for four weeks. The animals were sacrificed; the blood were collected; the blood glucose was measured; the autophagy relationship proteins (i.e., Atg5, LC3-I, LC3-II, Atg12) and apoptosis proteins (i.e., Beclin1, caspase3, cleaved caspase3, Bcl2, P-Bcl2) were...

Lethal autophagy is a pathway leading to neuronal death caused by transient global ischemia. In this study, we examined the effect of Ginsenoside Rb1 (GRb1) on ischemia/reperfusion-induced autophagic neuronal death and investigated the role of PI3K/Akt. Ischemic neuronal death in vitro was induced by using oxygen glucose deprivation (OGD) in SH-SY5Y cells, and transient global ischemia was prod...

Beclin 1 is a key factor for initiation and regulation of autophagy, which is a cellular catabolic process involved in tumorigenesis. To investigate the role of alternative splicing of Beclin1 in the regulation of autophagy in leukemia cells, Beclin1 mRNA from 6 different types of cell lines and peripheral blood mononuclear cells from 2 healthy volunteers was reversely transcribed, subcloned, a...

Autophagy is one of the main types of programmed cell death different from apoptosis, and it is a catabolic process involving the degradation of cell’s own components (damaged macromolecules and organelles) through lysosomal machinery. The main role of this process is to maintain the balance between the synthesis, degradation, and subsequent recycling of cellular products. As great attention ha...